Briosurfactant Case Study

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Isolation and Characterization of Biosurfactant produced by Pichia sorbitophila WG1 isolated from Grapes 1Vidur Bhatia, *Baljeet Singh Saharan1 and 2Deepansh Sharma 1Department of Microbiology, Kurukshetra University, Kurukshetra, INDIA 136 119 2Department of Biosciences, Lovely Professional University, INDIA 144 11 * Abstract Keywords: Biosurfactant, Glycolipids, Structural properties, Surface Tension, Yeast 1. Introduction Biosurfactants are amphiphilic molecules possessing hydrophilic and hydrophobic moieties that reduce surface and interfacial tensions by hoarding at the interface between two immiscible fluids (Saharan et al., 2011). Biosurfactants are a group of microbial products for biotechnological and industrial applications (Saharan et al., 2011; Banat et al., 2010; Desai and Banat 1997; Bodour and Maier 2002). They have various advantages above the synthetic surfactants, like low toxicity, better biodegradability foaming ability,…show more content…
After 72 h, cells were harvested by centrifugation (10,000 g, 10 min, 4°C) and the suspension was used to isolate the biosurfactant by ethyl acetate solvent extraction. The extracted biosurfactant was dialyzed against demineralized water at 4°C in a dialysis membrane (molecular weight cutoff 6,000-8,000 Dalton, Himedia, India) and freeze dried. 2.4. Structural characterization 2.5. Thin layer chromatography (TLC) The composition of the biosurfactant was determined by TLC followed by post chromatographic detection (Syldatk et al. 1985; Schenk et al. 1995; Hormann et al. 2010). The polysaccharides moities were stained with Syldatk reagents (anisaldehyde: sulfuric acid: glacial acetic acid 0.5:1:50). Plates were heated at 120 °C for 10 min. 2.6. Fourier transform infrared spectroscopy (FTIR) and Nuclear magnetic resonance (NMR)

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