Cellular Respiration Lab Report

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Cellular respiration is a process that most living organisms undergo to create and obtain chemical energy in the form of adenosine triphosphate (ATP). The energy is synthesized in three separate stages of cellular respiration: glycolysis, citric acid cycle, and the electron transport chain. Glycolysis and the citric acid cycle are both anaerobic pathways because they do not need oxygen to form energy. The electron transport chain however, is aerobic due to its use of oxidative phosphorylation. Oxidative phosphorylation is the process in which ATP molecules are produced with the assistance of oxygen molecules (Campbell et al 2008). Fermentation is a process adopted, typically, by anaerobic organisms to obtain ATP without the use of oxygen.…show more content…
Glycolysis inhibitor NaF can bind to the active site of the enzyme enolase which converts pholophoglycerate to phosphoenolpyruvate. This will cease fermentation and carbon dioxide production. The rate of fermentation will reach its maximum when under a certain temperature called optimum temperature. Further increase in temperature will denaturize enzyme protein and decrease the rate of activity. When we varied the fermentation temperature, we were using the same sugar which was sucrose, and other parameters were kept the…show more content…
No sugar was added 2. 1ml of glucose was added 3. 1ml of fructose was added 4. 1ml of sucrose was added 5. 1ml of arabinose was added 6. 1ml of sucrose and 1ml of NaF was added 7. 1ml of sucrose was added ( same as #4 but was incubated at room temperature) Second step was preparing fermentation chambers and candle jars: 1. Each tube was inverted several times by covering with parafilm to complete the mixture 2. A vial was placed over the upright held test tube and pressed firmly (fermentation chamber). 3. The fermentation chamber was inverted quickly and smoothly to obtain the smallest possible air bubble in the test tube 4. The estimated volume of the air bubble was recorded 5. #1-6 chambers were placed in the first candle jar and #7 was placed in the second candle jar 6. The candle in both jars was lighted, and both Mason jars were caped tightly 7. The timer was started up as soon as the light gone off 8. The first Mason jar was placed in the 37°C bath and the second one was left under room temperature 9. The amount of CO2 involved (ml) in each test tube was recorded at 10 minute intervals for a total of one

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