Cellular Respiration Lab Report

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Saul Gonzalez December 7th, 2011 Dr. Mark Birnbaum Biology Lab ATP Synthesis in Bovine Muscle and Liver Cells Cellular respiration occurs when mitochondria increases its production within the cell which include glycolysis, the citric acid cycle and oxidative phosphorylation. Cellular respiration produces adenosine tri-phosphate (ATP). Cellular respiration provides food for the cell as well. Glycolysis occurs in the cytosol outside of the mitochondria, which breaks down glucose into two molecules of glyceraldehyde-3-phosphate in which two ATP’s are to start the process. NAD+ picks up hydrogen electron ions in which NADH is made. Two-adenosine di-phosphate’s (ADP) attach to the G3P creating two ATP’s. Another set of reactions occurs and the three-carbon compound is rearranged and another set of two ADP’s react with the newly arranged three-carbon compound creating two more ATP’s. In total the output for ATP’s in Glycolysis are two ATP’s yielding two molecules of pyruvate. The citric acid cycle occurs only when the NADH are first converted into Acetyl-CoA. Acetyl-CoA splits and only the two…show more content…
Oxidative phosphorylation is the main output of up to 34 ATP while glycolysis and the citric acid cycle have a max output of 2 ATP each respectively, this is due to substrate level phosphorylation, which occurs in the cytoplasm in the cell. Glucose is converted into pyruvates, which are then oxidized and used in the citric acid cycle. Oxidative phosphorylation occurs in the inter-membrane space when electrons pass the electron transport chain. Cellular respiration is needed in order to allow for the movement of cell and energy to divide the cell. When separating sub particles of the cell, the mitochondria specifically from the supernatant that existed. Our hypothesis stated that bovine liver tissue would use the mitochondria more efficiently than bovine muscle tissue. (Campbell Biology,

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