Cellular Respiration Lab Report

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Cellular respiration is the process in which cells produce energy by using oxygen to break down glucose in order to store its energy in adenosine triphosphate[1] (ATP); however the rate of this reaction varies dependent on the environment in which the reaction takes place. So this experiment was derived to measure just how significant the changes are by exposing the yeast to various environments. A 50ml beakers was filled with 15ml of either 0.2M sucrose, 0.2M glucose, or a saturated starch then 0.5g of yeast was added to the solution then the solution was placed into a fermentation tube and depending on the condition that was assigned the fermentation tube would be placed either in an incubator (37oC), refrigerator (4oC), or placed in room temperature for 40 minutes while recording the milliliters of co2 produced in 5 minute intervals.…show more content…
So this experiment was done to verify that without the oxidation of succinate then cellular respiration was not possible. 6 cuvettes were labeled B-1,B-2,1,2,3,4 and filled with a buffer solution, DPIP, mitochondrial suspension, and succinate based on table 9.1, then the cuvettes were covered in parafilm, shaken then placed in the spectrophotometer to read the %transmittance and this step was repeated for all 6 cuvettes for 30 minutes each in 5 minute intervals. Abstract: summary of

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