Catalase Lab Report

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Earlier, it was stated in the hypothesis for Part A that if the pH level was too acidic or too basic, then the reactivity and reaction rate of which the catalase breaks down hydrogen peroxide will decrease. The hypothesis was true. There was no reaction when hydrogen peroxide was added to water because there were no catalase to break down the hydrogen peroxide. When H₂O₂, hydrogen peroxide, was added to the liver puree in test tube 1, 2 millimeters of bubbles were formed. When H₂O₂ was added to the liver puree in test tube 2, 110 millimeters of bubbles were formed, because there was less acid in it than test tube 1. However, when H₂O₂ was added to the liver puree in test tube 3, 124 millimeters of bubbles were formed further supporting my hypothesis,…show more content…
What was the purpose of test tube number 3 in procedure A? Test tube 3 served the purpose of being the control group in the lab. Without this, it would be impossible to determine wether acids or bases sped up or slowed down the enzymes reaction with the hydrogen peroxide. It was neither acidic (HCl) nor basic (NaOH). 2. How does temperature and pH affect the reaction rate of catalase? The temperature and pH affect the reaction rate of catalase immensely. Temperature effects the catalase's reaction speed. When temperature is too hot or too cold in relation to room temperature, a decrease in enzyme activity is observed. The liver catalse’s optimal temperature is room temperature. If the optimal temperature is exceed to the extreme, the enzyme will be denatured and become useless. A denatured enzyme means an enzyme’s shape will be changed, which results in a higher activation energy and the loss of function in an enzyme. The pH also affects the catalase’s reaction and the same concepts apply. Most catalase have an optimal pH level needed to achieve its maximum activity. If the pH level of its reactant is increase or decrease, a negative slope in the catalase’s reaction speed and quantity will be

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