Biology 101 Lab, Section 035 Dr. Elina Levina; TA: Eilea Knotts Catalase Enzyme Activity Abstract The human body utilizes catalase enzymes to break down hydrogen peroxide (Gaetani et al, 1994). This catalase enzyme system is important because it gets rids of toxins in hydrogen peroxide the body does not need and at a moderate rate (Kampshmidt, 1965). In this experiment, two hypotheses were tested to show how differences in substrate, or H2O2 concentration and differences in catalase, or phosphate
Mixed Unknown Bacteria Lab Report Biology 2460-002 Jamuna Kadariya November 24, 2014 Unknown number: thirty-four I. Abstract There are many types of bacteria and they produce different enzymes. However, some bacteria can produce same enzymes. In order to identify the particular enzymes produce by each bacteria specific test needs to be done. Among various types of enzyme producing bacteria, gram- negative and gram-positive bacteria are given priority in our experiment. There are four main types
Unknown Number 16 – Salmonella typhimurium Cindy Chai Rutgers University Microbiology for the Health Sciences Abstract This lab report was performed to identify the unknown organism, number 16, through various laboratory tests and techniques. The laboratory tests generated some critical results that suggested the unknown culture was Salmonella typhimurium. Some of the tests that led to the identification of the unknown organism included: the EMB agar plate that resulted as negative for lactose
for the evolution of gas. Result Evolution of a gas (Oxygen) causes bubbles – Positive No evolution of gas – Negative OXIDASE TEST Cytochrome oxidase is an enzyme found in some bacteria that transfers electrons to Oxygen, the final electron acceptor in some electron transport chains. Thus, the enzyme oxidises reduced cytochrome C to make this transfer of energy. Presence of cytochrome oxidase can be detected through the use of Oxidase Discs, which acts as an electron donator