needed to begin the reaction and speeding up the process to ensure it happens at a rate useful to human life and routine. There are hundreds of different enzymes in your cells, each responsible for specific reactions. The one studied in this lab is catalase, which accelerates the breaking down of the toxic chemical hydrogen
Concentration and Temperature on Catalase Activity Abstract: Catalase, an enzyme found in many living organisms, speeds up the decomposition reaction of hydrogen peroxide to prevent the formation of free oxygen radicals that can destroy tissue and organs. Many factors influence the function of catalase, including temperature and substrate concentration. Varying concentrations of hydrogen peroxide substrate solution (0.8%, 0.4%, 0.2%, 0.1%, and 0.0%) were reacted with a catalase solution, and the volume of
Earlier, it was stated in the hypothesis for Part A that if the pH level was too acidic or too basic, then the reactivity and reaction rate of which the catalase breaks down hydrogen peroxide will decrease. The hypothesis was true. There was no reaction when hydrogen peroxide was added to water because there were no catalase to break down the hydrogen peroxide. When H₂O₂, hydrogen peroxide, was added to the liver puree in test tube 1, 2 millimeters of bubbles were formed. When H₂O₂ was added to the
Research Question: How does the temperature (+/- 0.5˚C) of Hydrogen Peroxide (+/- 0.5cm3) and Catalase solution (+/- 0.5cm3) affect the amount of gas in a gas syringe when measured using a thermometer (+/- 0.5˚C)? Hypothesis: I predict that as the temperature increases, so will the rate of reactions of enzymes. The reason I predict this is because scientific research has shown that by increasing temperature increases the kinetic energy that molecules possess ("Factors affecting Enzyme Activity")
Discussion and Conclusion The results of the Catalase Enzyme Activity Lab were almost as hypothesized at the beginning of the experiment, but not exactly. From the average results of all eight groups, it is obvious that the percentage of substrate present in the solution is essential to the rate of reaction. While not all of the groups got the same ml 02/min, with a 0.8% concentration, the average of all of the group was higher for 0.8% concentration (30.54) then it was for 0.6% concentration (12
Effect of Varying Substrate Concentration and Temperature when Interacting with Catalase Buffer Solution Abstract Catalase enzyme is a catalyst found in living organisms that decomposes hydrogen peroxide into hydrogen and oxygen. It does this in order to protect cells from the harmful effects of hydrogen peroxide. In this experiment, we mixed different concentrations with the catalase enzyme, as well as mixed a 0.8% substrate concentration with differing temperatures of the enzyme. We hypothesized
Biology 101 Lab, Section 035 Dr. Elina Levina; TA: Eilea Knotts Catalase Enzyme Activity Abstract The human body utilizes catalase enzymes to break down hydrogen peroxide (Gaetani et al, 1994). This catalase enzyme system is important because it gets rids of toxins in hydrogen peroxide the body does not need and at a moderate rate (Kampshmidt, 1965). In this experiment, two hypotheses were tested to show how differences in substrate, or H2O2 concentration and differences in catalase, or phosphate
healthy body. In this experiment, the floating disk method was used to observe how catalase enzyme activity was affected by the concentration of substrate. Ultimately, this imitates the reactions that occur in the human body when the catalase enzymes break down and balance the hydrogen peroxide. A small disc of filter paper, which had been soaked in yeast, was placed into a hydrogen peroxide solution. The yeast catalase then reacted with the hydroxide peroxide, producing oxygen and water, presented
Mixed Unknown Bacteria Lab Report Biology 2460-002 Jamuna Kadariya November 24, 2014 Unknown number: thirty-four I. Abstract There are many types of bacteria and they produce different enzymes. However, some bacteria can produce same enzymes. In order to identify the particular enzymes produce by each bacteria specific test needs to be done. Among various types of enzyme producing bacteria, gram- negative and gram-positive bacteria are given priority in our experiment. There are four main types
Unknown Number 16 – Salmonella typhimurium Cindy Chai Rutgers University Microbiology for the Health Sciences Abstract This lab report was performed to identify the unknown organism, number 16, through various laboratory tests and techniques. The laboratory tests generated some critical results that suggested the unknown culture was Salmonella typhimurium. Some of the tests that led to the identification of the unknown organism included: the EMB agar plate that resulted as negative for lactose