Catalase Temperature Lab Report

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Research Question: How does the temperature (+/- 0.5˚C) of Hydrogen Peroxide (+/- 0.5cm3) and Catalase solution (+/- 0.5cm3) affect the amount of gas in a gas syringe when measured using a thermometer (+/- 0.5˚C)? Hypothesis: I predict that as the temperature increases, so will the rate of reactions of enzymes. The reason I predict this is because scientific research has shown that by increasing temperature increases the kinetic energy that molecules possess ("Factors affecting Enzyme Activity"). Doing so in a fluid creates more collisions between molecules. This result of more collisions has to do with the increase in velocity and kinetic energy that has come from the increase in temperature ("The Effects of Temperature on Enzyme…show more content…
2) Gather all safety equipment needed for this experiment and wear them. 3) Pre-heat the water bath to 60˚C. 4) Take the retort stand and place it on top of the table. 5) With the gas syringe, attach it to the retort stand using the screws on the side of the stand. 6) Make sure the gas syringe is straight and fasten tightly enough. 7) Taking the catalase solution and hydrogen peroxide, place one pipette in each of the glasses contain the liquid. 8) Using a marker, mark the pipettes so you can identify which belongs in what solution/peroxide. THEY CANNOT BE MIXED UP FOR ACCURATE RESULTS 9) Using a pipette, squirt 2cm3 of hydrogen peroxide into one of the measuring cylinders. 10) Using the other pipette, squirt 1cm3 of catalase solution into the 2nd measuring…show more content…
By now the water bath should have reach 60˚C. 12) The room temperature should be anywhere from 20-30˚C. If the temperature is 25˚C and above, place both cylinders inside the water bath until they reach 30˚C. If the temperature is 24˚C or below, add some ice cubes until it reaches 20˚C. 13) Once the temperature is reached, quickly mix the two liquids into a measuring beaker, and put the gas syringe top over the beaker. 14) Start the timer set for 3 minutes. 15) Create a table in your notebook of the different temperatures and how much gas there was for each temperature. 16) After 3 minutes record the amount of cm3 of gas as trial 1 for that temperature. 17) Wash out the beaker, and use the 2nd one that hasn't been used yet. 18) Repeat steps 12-16 until you have 5 trials for that temperature. 19) Depending on the temperature you will be measuring next, depends on the duration of how long the liquids will need to be in the water bath or how much ice will be needed. 20) Make sure to use the same amount of hydrogen peroxide and catalase solution to try and produce accurate enough
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