Dark Chocolate Experiment

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The researches performed this study to test the effect of dark chocolate on graft atherosclerosis. Within this study, dark chocolate was tested to see if the flavonoids can benefit health in heart transplant patients. To do so, Flammer et al. conducted a study in which 22 heart transplant recipients (18 men 4 women) were placed into a double-blind study in which they were given either dark chocolate or a control chocolate. The chocolate in this study was made by Nestle located in Lausanne, Switzerland. The dark chocolate was their “Nestle Noir Intense” and the control chocolate was specially made for this test. The only difference between the two chocolates is that the control lacked cocoa liquor which gives dark chocolate catechin and epicatechin in concentrations of 0.27 and 0.9 mg/g respectively. The patients were told to refrain from flavonoid rich food for 24 hours and to fast before the baseline test was conducted. They were tested before eating the chocolate then 2 hours after. For the tests, they evaluated the coronary arteries, shear stress, TRAP, Frap, and F2-isoprostane. The author considered a probability…show more content…
The polyclonal antibodies that bind to 8-iso-PGF2α makes a yellow color. The intensity of the yellow correlates with the amount of 8-iso-PGF2α within the sample. The 8-isoprostane levels in the dark chocolate group were significantly lowered 2 hours after eating the dark chocolate. Concentration of 8-iso-PGF2α before (pg/ mL) Concentration of 8-iso-PGF2α after (pg/ mL) Probability Value (P) Dark Chocolate 1212±536 829±341 0.029 Control Chocolate 1074±411 1002±393

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