Cell Culture Case Study

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APPLICATIONS OF CELL CULTURE Cell culture is one of the major techniques used nowadays in many applications, since it has a significant role in research, production and improvement of many products. Cell culture provides model systems being used for the study of the physiology and basic biochemistry of cells such as aging and cellular metabolism, for the study of effects of new drugs on cells, and to study mutagenesis as well as carcinogenesis. Animal cell culture technique can be used to study the differences between normal and cancer cells, to replace damaged tissue or organs, and to introduce new DNA or RNA into the cell in order to study the expression of the new gene. This technique can also be used in gene therapy that can help…show more content…
One of the major problems is the “Black Market” in cell lines, which provides cell lines with low cost, but these cell lines may be old and cross-contaminated. This problem was overcome by establishing cell banks and culture collections or any other reliable source, that are excellent sources of cell lines that have been tested for identity and contamination, to make sure that they are matching the original description and free of…show more content…
In order to estimate the cost required to start a cell culture, it is very important to consider the costs of cultivating cells, labor and time. Time-related costs are those spent on setup, sampling, product qualification, analysis, scale up, and cleanup. Other requirements are the basic equipment (laminar-flow hood, microscopes, incubators, autoclaves, and liquid nitrogen), additional equipment such as cells, vessels (disposable flasks, and multiwall plates), and other supplements such as media, serum, vitamins, hormones, and amino acids, as well as other reagents (Balanced salt solution, buffers, glucose, antibiotics, and mycoplasma kits). Since performing mammalian cell lines for the purpose of protein production are very expensive, researchers can use insect cells to produce such proteins, which are less in cost. Any cell culture costs can be reduced by lowering the serum levels being used, since serum is the most expensive additive used in cell culture

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