This study aimed to evaluate the possible anticancer effects of some natural venom compared with a synthetic anticancer drug 5-Flurouracil (5-FU) as a positive control. The evaluation parameters include cell viability, oxidant/antioxidant system analysis and the expression of some genes related to apoptosis by RT-PCR technique.
The oxidant/antioxidant system was assessed by measuring lipid peroxidation end-product malondialdehyde (MDA), nitrite/nitrate levels by product of NO and glutathione peroxidase (GPX) enzyme in the cancer cell lines as oxidative stress marker.
SRB assay of cytotoxicity:
In order to evaluation the cellular responses in MCF-7 and Caco-2 cells. The cells were treated with Naja h. venom, Leurius q. venom, Apis m. venom and 5-FU at concentrations ranging from 0.0µg/ml to 100µg/ml for 48 hours. The viability % and IC50 of each concentration was calculated in tables (5 to 9) and the IC50 data has been summarized at table (10)
1.…show more content… In MCF-7 case,
The highest percentage of toxicity was observed Apis m. venom followed by Naja h. venom, finally Leurius q. venom and compared with 5-FU toxicity.
2. In Caco-2 case,
3. The highest percentage of toxicity was observed Apis m. venom followed by Naja h. venom, finally Leurius q. venom and compared with 5-FU toxicity.
Table (): The % viability of MCF-7 cells post treatment with different concentration of Naja h. venom, Leurius q. venom and Apis m. venom and compared with 5-FU for 48 hours.
Concentration µg/ml Viability % of cells treated with 5-FU Viability % of cells treated with Apis m. Viability % of cells treated with Naja h. Viability % of cells treated with Leurius