Tazarotene Case Study

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Methods Compatibility Studies The organoleptic parameters were analyzed250. The tazarotene and glyceryl behenate mixture was characterized to confirm there is no reaction and the drug is intact with Differntial Scanning Calorimetry (DSC), infrared Fourier transform spectroscopy (IR), X-ray powder diffraction (PXRD) and Proton Nuclear Magnetic Resonance (H1-NMR). The physical mixture after the storage time was examined for organoleptic character251. The physical mixtures were prepared through simple mixing, in an amber glass vial, and kept for storage at 25°C ±2°C and relative humidity RH of 60%±5% for 3 months. Differential Scanning Calirometry (DSC) The thermal analysis of drug and lipid in 1:1 concentration was carried out by DSC 822e,…show more content…
Stock solutions of tazarotene were prepared in the solvent by dissolving 5 mg of the drug in 50 ml of the solvents mixture (100.0 µg/ml; n=6). The stock solutions were diluted serially to obtain the concentrations ranging from 0.5, 1, 1.5, 2, 2.5 and 5.0 µg/ml and were analyzed spectrophotometrically at λmax of 349 nm, using respective solvent mixture as blank. The observed absorbance for the respective dilutions were plotted against corresponding concentrations and straight line passing through origin was plotted. The extinction coefficient, of the drug was then…show more content…
Tazarotene was dissolved in the melted lipid phase prior to dispersion in the surfactant solution to obtain clear solution. The surfactant components Tween 80 and or Poloxamer 188 were mixed as per the required proportion in distilled water at room temperature, this surfactant dispersion (ie, distilled water with surfactant) was heated to the temperature of the lipid melt. The hot lipid phase was emulsified in the dispersion medium by high speed stirring at 8000 RPM with High speed homogenizer for one minute at a temperature 5 °C above the melting point of the lipid. This dispersion was then subjected to high pressure homogenization using homogenizer in Emulsiflex C3, Avestin at the optimized parameters, 1200 bars and 3 cycles. The obtained nanodispersion was allowed to cool to room temperature, forming lipid nanoparticles by recrystallization of the dispersed lipid. In different formulations the concentration of the surfactants were optimized. After cooling the dispersion was kept in refrigerator at 4-7°C in a well closed and covered bottle for protection against light. Blank-SLN (unloaded) were prepared as control using the same procedure with equal concentration of both the

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