In this lab we used different specimens and hydrogen peroxide to figure out the reaction rate for the enzyme catalase in that specimen. There were four different parts to this experiment. Part A consisted of Observing Normal Catalase Reactions. Part B was What Tissues Contain Catalase and Part C was titled What is the Effect of Temperature on Catalase Activity? And our final part was Part D and we found What is the Effect of pH on Catalase Activity. In Part A, Observe Normal Catalase Reaction,
The specific enzyme being observed in this lab was catalase which breaks down hydrogen peroxide (substrate) into water and the gaseous state of oxygen (product). Due to the fact that there were two experiments completed to measure the changes in the reactions of two different factors (substrate concentration and temperature), there were also two different hypothesis and null hypothesis. The first hypothesis states that the higher percentage of substrate will increase the reaction rate as well. For
Research Question: How does the temperature (+/- 0.5˚C) of Hydrogen Peroxide (+/- 0.5cm3) and Catalase solution (+/- 0.5cm3) affect the amount of gas in a gas syringe when measured using a thermometer (+/- 0.5˚C)? Hypothesis: I predict that as the temperature increases, so will the rate of reactions of enzymes. The reason I predict this is because scientific research has shown that by increasing temperature increases the kinetic energy that molecules possess ("Factors affecting Enzyme Activity")
Rates of Enzyme Catalase Abstract Catalase is a helpful enzyme in many organisms. It reacts when put in certain environments or mixed with certain solutions. The question at hand is what kinds of variables will make the enzyme react either faster or slower; essentially, what variables will make the reaction rate of catalase change. After adding factors such as hot water, acid, or hydrogen peroxide the results were somewhat predictable, the greatest reaction was found when the catalase was mixed with
Earlier, it was stated in the hypothesis for Part A that if the pH level was too acidic or too basic, then the reactivity and reaction rate of which the catalase breaks down hydrogen peroxide will decrease. The hypothesis was true. There was no reaction when hydrogen peroxide was added to water because there were no catalase to break down the hydrogen peroxide. When H₂O₂, hydrogen peroxide, was added to the liver puree in test tube 1, 2 millimeters of bubbles were formed. When H₂O₂ was added to the
Biology 101 Lab, Section 035 Dr. Elina Levina; TA: Eilea Knotts Catalase Enzyme Activity Abstract The human body utilizes catalase enzymes to break down hydrogen peroxide (Gaetani et al, 1994). This catalase enzyme system is important because it gets rids of toxins in hydrogen peroxide the body does not need and at a moderate rate (Kampshmidt, 1965). In this experiment, two hypotheses were tested to show how differences in substrate, or H2O2 concentration and differences in catalase, or phosphate
Concentration and Temperature on Catalase Activity Abstract: Catalase, an enzyme found in many living organisms, speeds up the decomposition reaction of hydrogen peroxide to prevent the formation of free oxygen radicals that can destroy tissue and organs. Many factors influence the function of catalase, including temperature and substrate concentration. Varying concentrations of hydrogen peroxide substrate solution (0.8%, 0.4%, 0.2%, 0.1%, and 0.0%) were reacted with a catalase solution, and the volume of
Effect of Varying Substrate Concentration and Temperature when Interacting with Catalase Buffer Solution Abstract Catalase enzyme is a catalyst found in living organisms that decomposes hydrogen peroxide into hydrogen and oxygen. It does this in order to protect cells from the harmful effects of hydrogen peroxide. In this experiment, we mixed different concentrations with the catalase enzyme, as well as mixed a 0.8% substrate concentration with differing temperatures of the enzyme. We hypothesized
healthy body. In this experiment, the floating disk method was used to observe how catalase enzyme activity was affected by the concentration of substrate. Ultimately, this imitates the reactions that occur in the human body when the catalase enzymes break down and balance the hydrogen peroxide. A small disc of filter paper, which had been soaked in yeast, was placed into a hydrogen peroxide solution. The yeast catalase then reacted with the hydroxide peroxide, producing oxygen and water, presented
Saam Baharmand Period 5 Enzyme Rate of Catalase Aim - The aim of this lab is to measure the rate at which Catalase breaks down hydrogen peroxide into oxygen gas and water. We can do this by recording the time it takes for a piece of filter paper dipped in catalase to float in H202. Purpose – Enzymes are biological catalysts that help speed up a reaction without affecting it. There are several enzymes in our bodies such as Lactase, Amylase, Catalase, and Pepsin. These enzymes help our organs with