Catalase Lab

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Saam Baharmand Period 5 Enzyme Rate of Catalase Aim - The aim of this lab is to measure the rate at which Catalase breaks down hydrogen peroxide into oxygen gas and water. We can do this by recording the time it takes for a piece of filter paper dipped in catalase to float in H202. Purpose – Enzymes are biological catalysts that help speed up a reaction without affecting it. There are several enzymes in our bodies such as Lactase, Amylase, Catalase, and Pepsin. These enzymes help our organs with thousands of everyday reactions in the body. When entered into the body, reactants or substrates bind to enzymes using the enzyme’s active site, and the enzyme breaks them apart into products. These products are then released and the enzyme is able to repeat the process. Catalase is an enzyme that breaks apart Hydrogen Peroxide (H2O2). Hydrogen Peroxide is a toxic waste product of cells that needs to be broken down. The H202 binds to Catalase and is separated into the harmless substances of Water and Oxygen gas. In this lab I will be examining the time (in minutes) it takes for a piece of filter paper dipped in catalase to…show more content…
Therefore there is an indirect relationship between the two variables. Variables - (Independent) – The concentration of the catalase enzyme in 5 different 20% intervals. This will be measured in milliliters ( i.e 20%, 40%, 60%, 80%, 100%). These settings and intervals were selected from the Catalase protocol I printed. I will measure these concentrations by using a dilution equation such as M1V1 = M2V2 where M stands for molarity and V for volume. Dependent – The time in minutes it takes for the filter paper to rise to the top of the Hydrogen peroxide and the distance the filter paper sinks and rises Fixed – 1) the amount of H202 is fixed at 20 ml. 2) The pH of hydrogen peroxide is kept constant throughout the

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