Amylase Lab Report

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Rula AbuKhalifa Partner: Ghalia Ghuneim 27 November, 2014 Biology G Mr. Lee Identifying Amylase in Stomachs with pH Levels & Enzymes Problem: Does amylase continue to break down carbohydrates in the stomach? Prelab: Control Group: • Positive control: Saliva • Negative control: Water Constants: • The same amount of water, saliva, and HCI added to each circle. • Same sprays of iodine on each side of the paper. Hypothesis: If we add hydrochloric acid to saliva, then the acid will affect the pH of the amylase in saliva because hydrochloric acid is a very corrosive acid that breaks down food in our stomach, and amylase is not present in our stomach. Materials: • Paper, big enough to draw in three circles. • Pencil • Saliva • Water • HCI (hydrochloric…show more content…
5/ Wait for ten minutes to let the substances dry. 6/ Once the substances have dried, spray two sprays of the iodine bottle on each side of the paper, and watch the colors of each circle. Table 1- Iodine Test Observations Data and Observations: 7/ Record your observations on a data table. Observations after Iodine Test Water Before it dried, the color was more clear and dark. Now it’s very dark blue/violet and not clear. Very small surrounding of light purple. Liquid is thin and slightly dampened the paper. Saliva Before and after it dried, it was a very light color, white outline/border with very light blue in the middle. White border is slightly spotted with brown spots from the iodine spray. HCl + Saliva Split in to two halves, top part is light purple and the bottom is very dark purple. Both halves are surrounded by a darker shade of purple, top half is very light purple with the dark purple border. The bottom half is dark purple with a slightly lighter purple surrounding it. Environment Color Positive/Negative for starch. Control…show more content…
3/ What were the positive and negative controls in the experiment? • The positive control in the experiment was saliva, because we used saliva knowing that it contained amylase. The negative control in the experiment was water. 4/ What is starch? Where is it found in nature? • Starch is a polysaccharide carbohydrate which contains a lot of glucose monosaccharides units that are mostly found in seeds and plants. Starch can be found in plants or fruits that have been naturally grown. 5/ How do animals obtain starch, why would they need it? • Animals obtain starch by eating grass or plants or foods that contain starch, or they feed on animals that have previously obtained food that contains starch. Animals need starch because they need energy, starch contains glucose that gives animals energy to function/move and survive. 6/ What does your saliva do to the starches? • Saliva contains amylase which breaks down carbohydrates in starch, since starch is made of many glucose monosaccharides. 7/ What was observed with the addition of hydrochloric acid? What did it do to the

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