Bread Lab Report

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Media composition: Fermentation broth was prepared using two different substrates. The composition of the culture broth is given below: Fermentation broth using waste bread as substrate: Waste bread 1.5% (NH4)2SO4 0.2% MgSO4.7H2O 0.04% KCl 0.1% KH2PO4 0.2% Yeast Extract 0.1% pH 6.5 Fermentation broth using waste of Citrus sinsencis as substrate: Waste fruit 1.5% (NH4)2SO4 0.2% MgSO4.7H2O 0.04% KCl 0.1% KH2PO4 0.2% Yeast Extract 0.1% pH 6.5 Media preparation: Using the given composition, 110 ml+110 ml culture broth was prepared in the flasks of 200 ml using both the substrates. The amounts of chemicals used were accordingly calculated. The pH was then maintained at 6.5 using the pH meter. After complete dissolution…show more content…
• 2- In one hand hold both the stock culture and the broth culture to be inoculated. Loosen the tube caps • 3-In your other hand hold the inoculating loop. • 4-Flame the inoculating loop to redness by holding it pointed down into the flame, starting near the handle and then moving the loop into the flame. This technique sterilizes the loop and, if wet with a culture, heats up the loop without spattering bacteria into the air and onto the surrounding area. • 5-Let the loop cool a minute. A hot loop will damage the bacteria cells. • 6-Using the fingers of the "loop hand" remove the cap from the stock culture tube and flame the tube mouth. DO NOT set the tube top down on the table. • 7-Insert the cooled sterilized loop into the culture tube being careful to not touch the sides of the tube. Touch the loop to the culture. You need not scrape a visible amount from the culture. Hold the tube as horizontal as possible to preclude particles from the air settling into the tube BUT do watch out for any condensate in the bottom of slant cultures. Don't let this fluid wash across the face of the culture. • 8-Remove the loop being careful again to not touch the tube…show more content…
The amounts of chemicals used were accordingly calculated. The pH was then maintained at 6.5 using the pH meter. After complete dissolution of various components it was tightly capped with the cotton plug. The media were then sterilized by autoclaving at 1210C; 15 psi pressure for 20 mins. Inoculum preparation: • 1-Light your Bunsen burner. • 2- In one hand hold both the stock culture and the broth culture to be inoculated. Loosen the tube caps • 3-In your other hand hold the inoculating loop. • 4-Flame the inoculating loop to redness by holding it pointed down into the flame, starting near the handle and then moving the loop into the flame. This technique sterilizes the loop and, if wet with a culture, heats up the loop without spattering bacteria into the air and onto the surrounding area. • 5-Let the loop cool a minute. A hot loop will damage the bacteria cells. • 6-Using the fingers of the "loop hand" remove the cap from the stock culture tube and flame the tube mouth. DO NOT set the tube top down on the
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