Alpha amylase is a protein enzyme. It is secreted by both the saliva and the pancreas. They It plays a very important role in the human digestive system. Glucose is one of the main sources of energy for the body, but the glucose molecule only forms part of larger molecules. One of the roles of digestion is to break these larger chains eg. Sucrose into individual glucose units which can then be used by the body. Alpha amylase hydrolysis the α-1,4 glucosidic bonds in large polysaccharides such as starch
Media composition: Fermentation broth was prepared using two different substrates. The composition of the culture broth is given below: Fermentation broth using waste bread as substrate: Waste bread 1.5% (NH4)2SO4 0.2% MgSO4.7H2O 0.04% KCl 0.1% KH2PO4 0.2% Yeast Extract 0.1% pH 6.5 Fermentation broth using waste of Citrus sinsencis as substrate: Waste fruit 1.5% (NH4)2SO4 0.2% MgSO4.7H2O 0.04% KCl 0.1% KH2PO4 0.2% Yeast Extract 0.1% pH 6.5 Media preparation: Using