To determine if changes in room temperature will have an effect on the activity of an enzyme Materials: -6 test tubes -Hot water bath -Liver -Test tube rack - Thermometer -Hydrogen Peroxide -10ml graduated cylinder - 3 250ml beakers -Distilled water -Tweezers -Ice bath Hypothesis: As temperature decreases, enzyme activity decreases until it is too cold for the enzyme to function. As temperature increases, enzyme activity increases until it is too hot for the enzyme to function and enzyme
Steven Bae Franckowiak AP Biology 26 September 2014 Enzyme Lab Introduction: Enzymes are proteins that speed up the rate of a certain chemical reactions. In the lab, the enzyme, catalase was studied. Catalase is essential to many living organisms. It helps convert hydrogen peroxide (H2O2) into water and oxygen. Hydrogen peroxide is powerful and not harmless, with any solution having more than eight percent hydrogen peroxide being corrosive to skin (Britannica). In everyday life, it can be used
Looking Into Lactase Purpose Why am I doing this lab? What am I trying to find out? The purpose of this lab was to see in which environments (temperature and pH levels), the Lactex® tablet would be effective (if at all). In the first section the purpose of the lab was to discover what type of milks the three milks were. The experiment is done on cow milk, rice milk, and soy milk. The importance of the next section was to see the effectiveness of the tablet in different pH levels. The second section
In order to use the pH meters in the lab foods must be in solution form, thus the tomato had to be mashed into juice and the wheat flour had to be a mixed with water to create a solution. The next technique used in this lab was titratable acidity; this measurement is used to measure the total amount of acid in a food, which helps food scientists to categorize a food and give them a profile to produce cheese or yogurt from milk. In this section of the lab a base 0.1 NaOH is used to neutralize a
5. Pre-lab and Post-lab questions: Pre-lab questions: 1. Since enzymes are mostly proteins, the monomers that compose enzymes are the amino acids. 2. Enzymes are biological catalysts that speed up a reaction. 3. The factors that affect the enzyme activity are: temperature, pH, ion concentration, allosteric interactions and inhibitors. 4. An extreme pH will denature the enzyme and disabling its normal activity because the enzyme’s conformation will change. Post-lab questions: 1. The
Research Question: How does the temperature (+/- 0.5˚C) of Hydrogen Peroxide (+/- 0.5cm3) and Catalase solution (+/- 0.5cm3) affect the amount of gas in a gas syringe when measured using a thermometer (+/- 0.5˚C)? Hypothesis: I predict that as the temperature increases, so will the rate of reactions of enzymes. The reason I predict this is because scientific research has shown that by increasing temperature increases the kinetic energy that molecules possess ("Factors affecting Enzyme Activity")
circumstances. In general, the effect of temperature of enzyme activity is that the colder the temperature the slower the chemical reactions and the warmer, the faster. The next enzyme activity was the effect of enzyme concertation. A higher enzyme concertation would yield a faster enzyme activity. Our last lab activity was the effect of pH on enzyme activity. Every enzyme has an ideal pH in which it will be the most
control group in the lab. Without this, it would be impossible to determine wether acids or bases sped up or slowed down the enzymes reaction with the hydrogen peroxide. It was neither acidic (HCl) nor basic (NaOH). 2. How does temperature and pH affect the reaction rate of catalase? The temperature and pH affect the reaction rate of catalase immensely. Temperature effects the catalase's reaction speed. When temperature is too hot or too cold in relation to room temperature, a decrease in enzyme
kingdom. Crickets are omnivorous, which signifies that they eat both plants and animals depending on what is accessible to them. In this lab, we investigated the effect of temperature on the respiration of crickets. Metabolic rate is the speed at which metabolism happens in a living organism. Endotherms use metabolic energy to sustain a steady body temperature comparative to their surroundings. They can create extra heat by increasing their metabolic rate when surroundings become chilly. When
Introduction L. variegatus, the species of sea urchin egg and sperm used in the HHMI Biology Lab, are found in the Atlantic Ocean and Caribbean Sea, flourishing in standard temperatures of 18℃-23℃ (Bryne 2007). Recently, climate change has become an increasing global issue, as industrial activities, primarily the burning of fossil fuels, produces CO2 gas that traps heat in the Earth’s atmosphere. This entrapment is causing the melting of ice caps, resulting in ocean cooling and warming in varied