whether or not enzyme concentration and temperature have an influence on enzyme rate of reaction. We feel that there is a direct correlation between environmental factors and enzyme activities. In order to test our hypothesis, an enzyme assay was performed at varying time intervals and temperatures. Our results showed that there was indeed a direct relationship between times. This is a very important discovery because this tells the science community the unique optimal conditions, which enzymes must work
independent techniques are also used to isolatate , identify and further understand the enzyme activity of the particular enzymes being studied and tested in the activated sludge process. Microbial activity of activated sludge can be analyzed by a culture independent technique known as metagenomics. One particular experiment took place in Hong Kong to study the gene structure and gene expression of the enzymes used in activated sludge. The present study applied both metagenomic and metatranscriptomic
body is a macromolecule, called an enzyme (Book). There are three categories of macromolecules including nucleic acids, carbohydrates and proteins. The macromolecule, the enzyme is classified under the protein category (http://www.austincc.edu/emeyerth/enzymes.htm). Enzymes are reaction specific meaning that they are the determinants of the process that will be carried out within a cell and when
The enzyme experiment The enzyme is a miraculous process necessary for many scientific explanations for life forms, and the overall existence of humanity. An enzyme, according to Geoffrey M. Cooper, is a “catalysts that [can] increase the rate of virtually all the chemical reactions within cells. Most biological reactions are catalyzed by proteins.” Thus the enzyme is made up of protein polypeptide bonds, and with the help of the enzyme, the chemical reaction can be sped up and occur within a few
Reacting at the Speed of Enzymes Alexandrea Cassidy Biology 1111 Section 7 Dr. Shazia Ahmed September 30, 2015 INTRODUCTION This experiment covered the role of enzymes in nature as catalysts to quicken reaction times and lower the energy required to begin a reaction (Ahmed 2005). The enzyme is considered a protein but it contains non protein parts named cofactors or coenzymes that are organic molecules. An enzyme is created in a specific shape with an area that is carved out called
Inhibitors slow down the process of enzyme activity. Enzyme inhibition has two types known as competitive inhibition and non-competitive inhibition. The substrate, inhibitor, active site, and enzyme play a major role/participate during a competitive inhibition. The inhibitor secures itself in the active site. This restrains the substrates from securing itself in the active site of the enzyme. The substrate, inhibitor, allosteric site, active site and enzyme participate in a non-competitive inhibition
catalyzed by the tyrosinase enzyme is exothermic. So using a microcalorimeter, the measured
The Effect of pH on the Performance of The Enzyme Tyrosinase Taelor Faulkner Madelyn Edwards, Sarahi Figueroa, & Chelsea Payan BIOL 1333 - 003, Shannon Beston March 2, 2015 The enzyme tyrosinase plays an essential role in food crops. This enzyme catalyzes tyrosine and eventually converts it to dopachrome (Bora de Oliveira et al. 2014). Tyrosinase is also accountable for melanin, a substance that provides color to eyes, hair, skin, and the browning of fruits and vegetables, and shielding the
Enzymes: Salivary Amylase and Phosphorylase – How are the directions of enzyme reaction affected by substrate concentration, reaction time and enzyme concentration Introduction Enzymes are biological catalysts made up of proteins, and they are used as an intermediate in chemical reactions without it, itself being used up. In this lab we are used two specific enzymes: salivary amylase and phorphorylase. Salivary amylase is a digestive enzyme that catalyses the hydrolysis of starch into sugars. It
research is always channelized to get novelty in enzyme or improvement of existing enzymes by engineering at gene and protein level [1]. Enzymes derived from microbial source are generally regarded as safe and they are functional at wide range of temperature, pH, salinity or other extreme conditions. Actinomycetes are one of the most diverse groups of microorganisms that are well characterized and recognized for their metabolic versatility. They play a vital role in decomposition of organic matter, e.g.