Enzyme Assay Lab Report

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Abstract The purpose of this experiment is to test whether or not enzyme concentration and temperature have an influence on enzyme rate of reaction. We feel that there is a direct correlation between environmental factors and enzyme activities. In order to test our hypothesis, an enzyme assay was performed at varying time intervals and temperatures. Our results showed that there was indeed a direct relationship between times. This is a very important discovery because this tells the science community the unique optimal conditions, which enzymes must work in. Introduction Enzymes are catalysts that speed up chemical reactions without being consumed by the reaction (Reece et al. 2011). Most enzymes are proteins that function to reduce activation…show more content…
Substrate and dye vii. Concentrated peroxidase 3) Adjust spectrophotometer to zero absorbance at 470 nm using tube 1. This tube is used so that any color in the reaction mixture will not influence following measurements when enzymes are present 4) One person on the team served as a recorder while another person served as a spectrophotometer reader and timer 5) Mix contents of tubes 2 and 3 by pouring them back and forth twice. This should be done within 10 seconds. Because no tube contains both enzyme and substrate, no reactions occur in the tubes as they are. One should be mixing pairs, as they are ready to measure. If mixed too early, the reaction will be over before measurements can be taken 6) Read out the absorbance at 20-second intervals from the start of mixing so that the recorder can write down values. After two minutes, remove the tube from the spectrophotometer and note color change. Discard solution properly. 7) Next, mix contents of tubes 4 and 5, and repeat step 6 8) After discarding previous reaction mixture, mix tubes 6 and 7. You will repeat step 6 once again. 9) In order to test the effects of temperature on peroxidase activity, you will repeat the enzyme assay with solutions warmed or cooled to four…show more content…
However, the activity of the enzyme at 48 ̊ C was about 1.5 times the activity at 32 ̊ C. All three had almost consistent slope. The activity of the enzyme with 1X enzyme concentration was a little higher than 1⁄2X but they were not significantly different. However, the enzyme activity with 2X enzyme concentration was twice the activity at 1X. There was no enzyme activity at a pH of 1 while pH 7 showed significant activity.
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