Activated Sludge Case Study

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Activated sludge is a particular process in which sewage and industrial wastewater are treated using oxygen and a biological floc composed of bacteria and protozoa. It is a very complex microbial system . The activated sludge process involves oxygen being inserted into a particular mixture of screened primary treated sewage or industrial wastewater that has combined organisms in which develop a biological floc which reduces the organic content of the sewage. Nowadays there is great interest being put into the research of activated sludge. This is due to the fact that the understanding of the fundamental microbial behavior has steadily increased throughout the years. One of the reasons is because of better engineered…show more content…
Problems related to the culture-dependent technique are well known. The results of the current study highlighted bias problems.(Al Awadhi 2013). The less known culture independent techniques are also used to isolatate , identify and further understand the enzyme activity of the particular enzymes being studied and tested in the activated sludge process. Microbial activity of activated sludge can be analyzed by a culture independent technique known as metagenomics. One particular experiment took place in Hong Kong to study the gene structure and gene expression of the enzymes used in activated sludge. The present study applied both metagenomic and metatranscriptomic approaches to characterize microbial structure and gene expression of an activated sludge community from a municipal wastewater treatment plant in Hong Kong.Enzyme subunits gene sequences annotation discovered that subunits of ammonia monooxygenase (amoA, amoB, amoC) and hydroxylamine oxygenase had higher expression levels compared with subunits of the other enzymes genes. Taxonomic profiles of selected enzymes (ammonia monooxygenase and hydroxylamine oxygenase) showed that ammonia-oxidizing bacteria present mainly

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