Calorimetric Continuous Assay Lab Report

946 Words4 Pages
. Instead of using a spectrophotometer to measure the change in pigment, a calorimetric continuous assay, which measures the change in heat, could have been used to monitor the progress of the tyrosinase reaction. The spectrophotometer used in this lab measured the change in the amount of pigment in the cuvette over time. Since more light was absorbed as the reaction progressed, the progress of the reaction could be measured using the absorbance levels obtained from the spectrophotometer. A calorimetric continuous assay measures the amount of heat released or absorbed by the reaction. All reactions involve at least some change in heat and the reaction catalyzed by the tyrosinase enzyme is exothermic. So using a microcalorimeter, the measured…show more content…
Enzymes are used extensively by foods and beverage companies to reduce costs and improve the overall quality of their products. Find two examples of enzymes used by the food and beverage industry. Explain their structure and function. Here is one site that might help you get started: One example of an enzyme used in the food and beverage industry is Rapidase. Rapidase is a fruit-processing enzyme. It maximizes extraction of juice from raw fruits and increases the clarity and natural color of the juice without affecting the taste. Rapidase works by decreasing the molecular weight of polysaccharides attached to the fruit cell wall, and reduces their binding. This enzyme is derived from a fungus called Aspergillius niger. Another enzyme that cuts costs and enhances the overall quality of food products is the enzyme called Maxiren. Maxiren optimizes cheese shredding and slicing by reducing proteolysis over shelf-life. It maintains cheese texture for an extended period of time so more production flexibility is offered. Since Maxiren has such a high specificity, it reduces the amount of coagulant required. This enzyme breaks down cheese proteins much slower than other coagulants. Maxiren is a bovine chymosin, a protease found in the stomach of cows that selectively cleaves the milk protein. This enzyme uses an aspartic reside to cleave the peptide bonds in

    More about Calorimetric Continuous Assay Lab Report

      Open Document