Gas Chromatography

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The methane production from the bottles were quantified using a Gas Chromatography (TraceTM 1310, Thermo Scientific), see in Figure 3a. In Appendix A a step-by-step procedure for the technical specifications regarding the GC machine and software used is presented. A calibration curve of known standards with specific methane concentrations was made before measuring the bottles. The bottles were sampled by using a Myjector U-100 insulin syringe with a pressure lock. The syringe was introduced through the rubber stopper and flushed by moving the plunger up and down at least three times. This was done to ensure equilibrium of methane between the bottle and syringe. A sample volume of 200 µL was adjusted and the pressure lock activated before removing…show more content…
The standard curve was made using four standard gases consisting of respectively 5%, 40%, 60% and 100% methane. The standards were always measured in triplicates starting from low to high concentrations. During each day of measurement the calibration curve was evaluated through a extra measurement with known methane concentration, a check standard. To further evaluate the accuracy of the standard curve, the use of two types of equipment were compared through statistical analysis of the measured methane illustrated by Figure 3b and…show more content…
2005). Through the TS analysis the dry matter content was quantified. The VS analysis revealed the content of organic material in the inocula. The analyses was performed in four-replicates. Each sample was dried in two stages. First the sample was heated for twenty four hours at 105 ◦C to evaporate all water. The initial sample weight was then compared with the sample weight after drying in order to determine the TS content. The dry sample was then ignited for two hours at 550 ◦C and weighed both before and after to quantify the VS content of the inocula. The TS and VS was calculated using Equations 10 and

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