Gas Chromatography Lab

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Introduction: Polychlorinated biphenyls, also known as PCBs are a family of congeners. In order to determine the extent of which contamination occurs further analytical techniques are needed. Each PCB consists of a biphenyl molecule with one to ten chlorines attached. Gas Chromatography is used to allow for separation of the PCBs. In this experiment, Gas Chromatography was performed in order to provide the necessary data needed for evaluation of PCB contamination. Materials & Methods: This experiment required the use of computers with a MSD Chemstation software. The samples were first acquired on a GC system equipped with a split injector, a mass spectrometric detector with an electron ionization source, a quadrupole analyzer, and autosampler.…show more content…
This mixture consisted of 21 individual PCB congeners. The retention times were determined by using the integration function on the software. Each peak was then numbered from 1 to 21. An interpretation of the mass spectra was then conducted by evaluating the isotopic pattern. The second peak and isotopic patterns of ions 256, 221, and 186 were evaluated. The differences in their abundance were observed and documented. Molecular mass difference was then calculated between those fragments and recorded. The mass spectra of each peak were obtained and the degree of chlorination of each peak was then recorded based on the calculated molecular weights of the PCBs. The Aroclor1254 file was evaluated second. The number 12 represents 12 carbon atoms in each molecule of PCBs while the number 54 is the percent of chlorination. In the chromatogram, all peaks were integrated and then labeled. The number of chlorines for each PCB was then determined by using the method of ion extraction through the software. The mass spectra for two tetra-chlorinated PCBs was then found and compared with one…show more content…
Chromatograms of Aroclor 1254 and the degraded Aroclor are also provided in the (JAS_lab8.ppt) file under slide thirteen. The isotopic patterns of ions 256, 221, and 186 show some height differences. There is a molecular mass difference of 35 between those fragments. The isotopic pattern can be attributed to A+1. There are some patterns present in the MS spectra when looking at different PCB congeners. The PCB congener retention times and extraction of their molecular weight ion show that as retention times increase, the degree of chlorination also increases. The extracted-ion chromatogram presents different colors to show a difference in the degree of chlorination. When the ion with a mass of 292 is highlighted, it corresponds to the four-chlorine compound. Based on observations between two mass spectra for two tetra-chlorinated PCBs on slides ten through eleven in the (JAS_lab8.ppt) file, one cannot distinguish between different PCB isomers. This could be because they isomers have the same properties, just in different

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