Enzyme Lab

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Enzymes are protein catalysts that speed up chemical reactions in living organisms. This experiment tested the effects of temperature and substrate concentration has on Enzyme activity. Catalase found in beef liver was treated with different temperature (~0o, ~25o, ~40o and 100oC) and substrate concentrations (.0.8%, 0.4%, 0.2%, 0.1%, 0.8%). Data was collected by measuring the amount of oxygen produced. The results gathered displays that catalase does not work at extreme temperatures and with low substrate concentrations. Introduction The use of enzymes to carry on life functions are required by cells. Functions of enzymes include breaking down molecules for energy and building structural molecules. Without the aid of catalysts, most reactions…show more content…
Then to fill the 10 ml graduated cylinder to overflowing with water. The opening of the graduated cylinder with the thumb, then it is to be inverted in the 600 ml beaker. It was essential for there not to be air bubbles in the graduated cylinder because having gas in the chamber would make the calculations inaccurate. After inverting the graduated cylinder, the short end of the U-shaped glass tube was inserted in the 600 ml beaker, placing the tip of the tube inside the 10 ml graduated cylinder. The rubber stopper is then placed in the opening of the 50 ml Erlenmeyer flask after adding 10 ml of substrate solution and 10 ml of catalase/ buffer solution to this reaction flask. It was noted that for all the experiments performed, the reaction chamber will contain 10 ml of enzyme/ buffer solution and 10 ml substrate solution. Upon adding catalase, it was suggested to swirl the flask gently, by holding it by the neck only, at a constant rate for all the experiments or the mixing rate would confuse the effect of other variables. As the reaction proceeded, O2 filled the graduated cylinder and the time it took to collect 10 ml of O2 in the graduated cylinder was recorded on the data sheet provided. These steps were taken which each of the different substrate concentrations, .8%, .4%, 0.2%, 0.1%, 0.0%, and then taken with the enzymes with temperatures, ~0o, ~25o, ~40o and 100oC, with the substrate concentrate

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