Enzyme Lab

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Outside Effects on the Rate of Reaction of the Catalase Enzyme By: Capria M Davis Bio 125A Lab 11am-1:50pm Dr. Erin Mulholland 10-28-14 Abstract In this experiment, the rate of reaction of a catalyzed enzyme is being tested under three different controlled variable circumstances. These variables include the amount of enzyme used, temperature as well as pH level. This is important because it allows us to look at how enzymes react when put under different conditions. This is especially useful when it comes to looking at reactions within the human body, where temperature and pH levels can be a big variable that enzymes within the body deal with. The three variables were tested by using a yeast cell, which held the enzyme,…show more content…
That is a question that was posed through this study. Why is the catalyzing of enzymes so important and how are they affected by different variables? How is the rate of reaction affected? Within this experiment these questions can be answered. This study takes a look at three different conditions for which the enzyme is placed within. The goal is to determine how the rate of reaction varies when each condition is applied. The three controlled variables are the concentration of the enzyme, the temperature of the enzyme, and the pH level of the solution that the enzyme is in. It is predicted that each of these three variables will indeed affect the rate of reaction, but how so? The prediction is the rate of reaction may speed up with an increased concentration of the enzyme, it may increase with the temperature increase, and it may stay neutral or unaffected by pH. But how exactly does this pertain to the daily life of a human being? As stated before enzymes are present within organisms (which humans are comprised of) and the conditions of our body may very well affect the conditions of the enzymes within us and in turn control how we function…show more content…
Within the human body it could be anything from too much enzyme concentration to a fever increasing the body temperature and therefor reducing or increasing the reaction rate of the bodily enzymes. It shows the importance of a very small factor and its dramatic effects. As seen from outside studies, if the enzyme catalyzing “system” within the human body gets out of control diseases and other ailments can take over. Without this understanding of how enzymes function under particular circumstances, we would not be able to medically figure out what is causing a problem nor how to fix it. Enzymes are important. Literature Cited Ene, M., & Maria, G. (2012). Temperature decrease (30–25°C) influence on bi-enzymatic kinetics of d-glucose oxidation. Journal Of Molecular Catalysis B: Enzymatic, 8119-24. doi:10.1016/j.molcatb.2012.05.001 Khodayari, S., Salehi, Z., Fakhrieh Asl S., Aminian, K., Mirzaei Gisomi, N., & Torabi Dalivandan, S. (2013). Catalase gene C-262T polymorphism: Importance in ulcerative colitis. Journal of Gastroenterology & Hepatology, 28(5), 819-822. doi:10.1111/jgh. 21141 Lubkowska, A. A., Dolegowska, B.B., Szygula, Z.Z, & Kilmek, A. A., (2009). Activity of selected enzymes in erythrocyes and level of plasma antioxidants in response to single whole-body cryostimulation in humansk. Scandinavian Journal of Clinical & Laboratory Investigaion,

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