Saam Baharmand Period 5 Enzyme Rate of Catalase Aim - The aim of this lab is to measure the rate at which Catalase breaks down hydrogen peroxide into oxygen gas and water. We can do this by recording the time it takes for a piece of filter paper dipped in catalase to float in H202. Purpose – Enzymes are biological catalysts that help speed up a reaction without affecting it. There are several enzymes in our bodies such as Lactase, Amylase, Catalase, and Pepsin. These enzymes help our organs with
using molecular models. To experimentally test the enzyme catalase in liver cells with hydrogen peroxide and identify the type of reaction occurring. To determine which types of tissue respond and react with catalase. To discover how the change in temperature affects enzyme (catalase) activity. To observe the effects of pH on enzymes. Background: In this lab, liver, muscle tissue, apple, carrot, and potato were used to examine the reaction between hydrogen peroxide and the enzymes in
healthy body. In this experiment, the floating disk method was used to observe how catalase enzyme activity was affected by the concentration of substrate. Ultimately, this imitates the reactions that occur in the human body when the catalase enzymes break down and balance the hydrogen peroxide. A small disc of filter paper, which had been soaked in yeast, was placed into a hydrogen peroxide solution. The yeast catalase then reacted with the hydroxide peroxide, producing oxygen and water, presented
The effects on rate of reaction of enzymes when changes in temperature, pH and enzyme concentration occur Abstract The purpose of this lab was to test the effect a change in temperature, pH and enzyme concentration has on the rate of reaction of enzymes. The enzyme being studied is known as catalase, and it is used to speed up the reaction that breaks down hydrogen peroxide into water and oxygen. Using enzyme rich liver and reacting it with solutions of various temperatures, pH, and concentrations
protein molecules that serve as catalysts. They themselves do not change during the reaction, but speed up or slow down reactions by breaking down a single substrate molecule into smaller ones or joining two or more substrate molecules together. Because of a unique amino acid sequence that forms a unique three-dimensional structure, each enzyme only acts upon a specific substrate during an enzyme catalyzed reaction. Enzymes are associated with both plant and animal metabolism; however, they have a
The Effects of pH Levels on Catalysts Samantha Burns September 29, 2015 Mr. Lau Abstract This lab’s purpose is to demonstrate the effect of pH on the catalase enzyme found in liver peroxisomes. It is hypothesised that if the pH level rises, then the rate of reaction will become faster. This will be determined by reacting hydrogen peroxide with liver peroxisomes in three different tubes with varying pH levels. The major findings from this laboratory were: The bubbles grew at a rate of about
Introduction The identification of a microorganism is not only vital in a microbiology lab, but also in the medical and pharmaceutical fields. Knowing the identity of a microorganism has helped diagnose numerous diseases, discover the source of the pathogen, and has provided patients with effective treatments such as antibiotics. In addition, the morphology of a bacterium and an arrangement of biochemical tests are utilized to identify an unknown bacterium. In this experiment, students performed
Unknown Number 16 – Salmonella typhimurium Cindy Chai Rutgers University Microbiology for the Health Sciences Abstract This lab report was performed to identify the unknown organism, number 16, through various laboratory tests and techniques. The laboratory tests generated some critical results that suggested the unknown culture was Salmonella typhimurium. Some of the tests that led to the identification of the unknown organism included: the EMB agar plate that resulted as negative for lactose