Yeast Lab Report

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The objective of this experiment is to calculate the exponential growth rate of Saccharomyces cerevisiae, a type of yeast. The yeast is being tested under two different liquid mediums where the first medium is the Sabouraud’s dextrose broth and the second is the Nutrient broth. The major difference between the two type of broths is the concentration of nutrients as the Sabouraud’s dextrose broth is rich in nutrients while the Nutrient broth contains fewer nutrients. The hypothesis of this experiment is that the logarithmical growth rate of the yeast cells in the Sabouraud’s dextrose broth will be higher than the yeast cells growing in the Nutrient broth due to the fact that the Sabouraud’s dextrose broth is rich in nutrients, therefore there…show more content…
Namely, this is the metabolism of yeast. The two fundamental pathways of the yeast metabolism are cellular respiration and alcoholic fermentation (Feldmann 25), which both occur after “glucose, the main source of energy production” (Feldmann 25) is converted to two pyruvates in the process of glycolysis and the “production of energy in the form of ATP is coupled to the generation of intermediates and reducing power in the form of NADH for biosynthetic pathways” (Feldmann 25). In the cellular respiration of yeast, the glycolysis reaction connects to the citric acid cycle as the two molecules of pyruvates “enter the mitochondrial matrix” (Feldmann. 25) and being converted to acetyl-CoA which eventually finalizes the “oxidation of glucose” (Campbell and Reece 170). On the other hand, “alcoholic fermentation” ( Barnett, J. A., and Barnett, L. 1), is a “conversion of sugar into carbon dioxide and alcohol in the absence of oxygen” (Barnett, J. A., and Barnett, L. 1). There are two steps involved in the alcoholic fermentation: the “release of carbon dioxide to convert to the two-carbon compound acetaldehyde” and “the reduction of acetaldehyde by NADH to ethanol” ( Campbell and Reece 178). Overall, the two main difference between cellular respiration and alcoholic fermentation of yeast is the “presence of oxygen”

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