two different substrates. The composition of the culture broth is given below: Fermentation broth using waste bread as substrate: Waste bread 1.5% (NH4)2SO4 0.2% MgSO4.7H2O 0.04% KCl 0.1% KH2PO4 0.2% Yeast Extract 0.1% pH 6.5 Fermentation broth using waste of Citrus sinsencis as substrate: Waste fruit 1.5% (NH4)2SO4 0.2% MgSO4.7H2O 0.04% KCl 0.1% KH2PO4 0.2% Yeast Extract 0.1% pH 6.5 Media preparation: Using the given composition, 110 ml+110 ml culture broth was prepared