macromolecule which functions as the most important encoder of genetic instructions for all currently known living organisms. DNA, just like RNA is a nucleic acid which consists of two polymer strands forming an anti-parallel structure called a double helix. (Figure 1.) These strands are built up from ribose molecules chained together with phosphate molecules in between them, forming the backbone of a DNA molecule. A single DNA-strand is approximately 2,2 to 2,6 nanometers
Denaturation The DNA template is heated to 94° C. This breaks the weak hydrogen bonds that hold DNA strands together in a helix, allowing the strands to separate creating single stranded DNA. 2. Annealing
into two categories; qualitative and quantitative analysis. Both types of analysis can be used to provide important information about an unidentified sample and help to identify what the sample is. Analytical chemistry covers almost all of the areas of chemistry, but involves the development of tools and methods to measure physical properties of substances and apply those techniques (qualitative analysis) and quantify the amount (quantitative analysis) of species in a wide variety. CCE presentations
CHAPTER-1 INTRODUCTION 1.1 SERUM ALBUMIN Among all the proteins present in blood plasma, Albumin is the most abundant extracellular protein. This is also the most extensively studied of all proteins accounting for about 57 - 60% of the total proteins, which corresponds to a concentration of about 42 g/L [1, 2]. It also provides about 80% of the osmotic pressure of the blood [1] and is used as a model protein for most of the biophysical, biochemical and physicochemical studies [2]. These proteins