that some principle of transformation had taken place to cause this occurrence. Fifteen years later Avery, McCloud, and McCarty discovered the natural process by which transformation occurs. They separated the genetic material from the virulent form of streptococcus and transformed the nonvirulent strain into a 100% virulent
genome, genetic transformation has taken place, bacterial transformation is a form of genetic transformation and also the easiest to create within a lab due to the single cell nature of the bacteria used. For this particular experiment pGLO plasmid will be incorporated into E. coli bacteria which will give it genes that code GFP and beta-lactamase proteins into the modified genome of the bacteria. For the experiment two plates were mixed with and without the pGLO plasmid; one was the pGLO, LB nutrient
celled organisms that play an important role in microbiology The use of the transformation procedure enables others to inserts various genes by recombinant techniques and to place the plasmid into a bacteria for a gene expression. In the lab experiment, the transformation of Escherichia coli with new DNA was explored to determine its different genetic information. In order to grow the bacterium the plate must contain a pGLO plasmid and must be expressing the ampicillin resistance protein β-lactamase
Bacterial Transformation Alyssa Krajnik Lab TA: Qi Deng Lab Time: Thursday 4:30 Report Submitted: 09 APR 2015 Abstract: The purpose of this experiment was to observe the process of transferring genes from one organism to another using the aid of a plasmid. However, the results obtained from the experiment illustrated that the data collected was not reliable. The results indicated that not enough bacteria were transferred to the agar plates. Because of the errors that occurred in the experiment