Pglo Transformation Lab Report

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The purpose of this experiment is to explore the understanding of bacterial transformation of plasmid DNA. Bacterial transformation is the technique of genetic transformation. In this lab the pGLO plasmid is pledged into E.Coli bacteria and adds genes encoded for GFP and beta-lactamase to transformed bacteria. To get the results of the experiment, the bacteria processed with plasmid were grown on two plates , one of which containing LB nutrient broth and ampicillin and another plate containing LB broth, arabinose and ampicillin. The other two plates were grown without plasmid to contrast the first two plates, one plate containing LB broth and ampicillin and the other plate containing just LB broth. The results showed that the colonies grown in the presence of the plasmid and in the absence of the plasmid. The transformation efficiency was much higher in the tests with – pGLO and without ampicillin, than in the tests with +pGLO.…show more content…
The transferred DNA then is expressed in the genetic information of new recipient. E.Coli is a bacteria. Ampicillin is an antibiotic that kills E.Coli and contains beta-lactamase. Plasmid is a circular DNA molecule, capable of self-replicating, carrying one or more genes for antibiotic resistance and a cloned foreign gene such as GFP. Plasmids usually occur naturally in bacteria. pGLO is a plasmid containing the Green Fluorescent Protein gene sequence and ampicillin resistance gene. Ampicillin is a well-known drug, which is widely used in medicine. Such infections as bladder infection, ear infection, pneumonia are treated with ampicillin. The bacteria used for this lab experiment was E.Coli, because it was ideal for transformation, since it grows easy in LB nutrient

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