Pglo Transformation Lab Report

1527 Words7 Pages
Abstract Bacteria are single microscopic celled organisms that play an important role in microbiology The use of the transformation procedure enables others to inserts various genes by recombinant techniques and to place the plasmid into a bacteria for a gene expression. In the lab experiment, the transformation of Escherichia coli with new DNA was explored to determine its different genetic information. In order to grow the bacterium the plate must contain a pGLO plasmid and must be expressing the ampicillin resistance protein β-lactamase. If arabinose is present, transcription of the gfp gene is enabled that results in the expression of the gfp protein. Transcriptional regulation DNA-RNA for the arabinose operon regulated the expression of…show more content…
Transformation is the process by which competent bacteria take up foreign DNA from the environment. In order for transformation to happen the bacteria must be competent. Competent cells are cells that incorporate foreign DNA such as Streptococcus pneumonia. The cells are made competent through heat shock and a calcium chloride ice bath. Artificial competence is when competence is induced, the transformation process didn’t occur naturally. Artificial transformation into E.coli involves the transfer of plasmid DNA. A plasmid is a small circular piece of DNA that transfers genetic information between different bacteria that allows the microbes to adapt to new environments and to gain antibiotic resistance (2). The plasmid pGLO encodes for the green fluorescent protein (gfp) derived from the jellyfish Aequorea victoria, a gene that carries an ampicillin resistance (bla) β-lactamase. Gfp is under the control of an arabinose inducible promoter, a gene that encodes for the transcriptional regulator (araC) and (ori) for an origin of replication. If the transformation is successful, the transformants will be resistant to ampicillin and gfp positive in the existence of arabinose. Arabinose is a carbohydrate isolated from plants used as a source of food by bacteria

More about Pglo Transformation Lab Report

Open Document