Pglo Transformation Lab Report

2540 Words11 Pages
INTRODUCTION In 1928 a man named Fredrick Griffiths discovered that cells could be transformed or contain different genes. He was working with a strain of streptococcus pnumoniae that was nonvirulent and a strain that was virulent form of streptococcus pnumoniae. He found that the nonvirulent strain that he injected into his test mice had been transformed by heat killed virulent streptococcus and the mice died. He theorized that some principle of transformation had taken place to cause this occurrence. Fifteen years later Avery, McCloud, and McCarty discovered the natural process by which transformation occurs. They separated the genetic material from the virulent form of streptococcus and transformed the nonvirulent strain into a 100% virulent…show more content…
The main source of GFP is Aequorea Victoria, a species of bioluminescent jellyfish. The GFP allows these jellyfish to glow in the absence of light. pGLO has specific regulations for genes, which are able to control fluorescent protein expression in transformed cells. This is done by using the sugar arabinose. When the araC protein binds to the DNA sequence and is turned on in the presence of arabinose and activates it and it helps the araC bind to the RNA polymerase and transcribes the araA, araB, and araD enzymes. The coding sequence of the pGLO has been modified to include many of the inner workings of the arabinose operon which uses arabinose as an inducer. The promoter (PBAD) and the araC gene are present in the process of translation. The genes that code for the catabolism of arabinose, araB, araA and araD, are replaced by a single gene which codes for the fluorescent protein in the cell. This proves that, with arabinose present, the araC protein allows for the binding of RNA polymerase and the translation of the fluorescent protein are…show more content…
DNA and RNA are separated by size only, and proteins are separated by size and charge. A gel is performed by running an electric current through the gel which runs from the anode to the cathode because DNA is negatively charged. The current moves the solution through the gel that shows the band size of the molecules, with larger sizes being near the top and vice versa for smaller bands. This was used with restriction enzymes to see where the restriction enzymes cut the pGLO plasmid DNA. A molecular weight ruler is used to give a guide to read the size of the bands being generated. The gel is observed in ultraviolet

More about Pglo Transformation Lab Report

Open Document