Enzymes: Chemical Degradation of Albumin through Light Absorbance Mariel Melchor, Itzae Facio, Sarahi Iturriaga, Sarai Chairez, Evelyn Tovanche University of Texas at El Paso BIOL 1107 October 9, 2014 Horacio Gonzalez Abstract In this enzyme lab, we worked to observe enzymatic activity by measuring how fast pepsin degrades albumin by observing the changes in absorbance of albumin using a spectrophotometer. The wavelength set through a spectrophotometer is the controlled variable
Effect of different temperatures on amylase activity. Literature review This study is an attempt to follow the activity of amylase because it has a major role in the life of living organisms and is found abundantly in them. Amylase is a catalytic enzyme which hydrolyzes starch into maltose and dextrin at a certain temperature (Biology.kenyon.edu, 2015). In plants such as fruits and vegetables carbohydrates are referred to starch which is polysaccharide and is converted into disaccharide and eventually
certain paralogs unique to certain strains? We will be researching duplicated genes of THC Synthase that recently became prevalent due to new sequencing abilities and an assembly of the cannabis genome given to our lab by Steep Hill Labs, Inc. We will be conducting our research is within the lab of Dr. Nolan Kane, under the supervision of Dr. Daniela Vergara and Dr. Nolan Kane. The Kane Lab’s
dream was to work in medical field, I always explored something that relates food, nutrients, and human health. Finally, I was pondering to read an article in 2010 in daily star heading ‘Toxic poultry feed poses health risk'. I came to know from the report that poultry and fish feed possessed by tannery waste which is a serious health risk for consumers because contaminants directly entering the food chain. After reading that article, I have a lot of question into my mind. What happen if heavy metals
Unknown Number 16 – Salmonella typhimurium Cindy Chai Rutgers University Microbiology for the Health Sciences Abstract This lab report was performed to identify the unknown organism, number 16, through various laboratory tests and techniques. The laboratory tests generated some critical results that suggested the unknown culture was Salmonella typhimurium. Some of the tests that led to the identification of the unknown organism included: the EMB agar plate that resulted as negative for lactose
Mixed Unknown Bacteria Lab Report Biology 2460-002 Jamuna Kadariya November 24, 2014 Unknown number: thirty-four I. Abstract There are many types of bacteria and they produce different enzymes. However, some bacteria can produce same enzymes. In order to identify the particular enzymes produce by each bacteria specific test needs to be done. Among various types of enzyme producing bacteria, gram- negative and gram-positive bacteria are given priority in our experiment. There are four main types
Media composition: Fermentation broth was prepared using two different substrates. The composition of the culture broth is given below: Fermentation broth using waste bread as substrate: Waste bread 1.5% (NH4)2SO4 0.2% MgSO4.7H2O 0.04% KCl 0.1% KH2PO4 0.2% Yeast Extract 0.1% pH 6.5 Fermentation broth using waste of Citrus sinsencis as substrate: Waste fruit 1.5% (NH4)2SO4 0.2% MgSO4.7H2O 0.04% KCl 0.1% KH2PO4 0.2% Yeast Extract 0.1% pH 6.5 Media preparation: Using
(positive control for the ELISA, substance to immunise the animals in step two), ii) Immunisation of a sheep with the IL-1β antigen, iii) Immunisation of mice with IL-1β and production of monoclonal antibodies, iv) Establishment of a sandwich ELISA (Enzyme Linked Immunosorbent Assay) with the antibodies and the antigen. These steps were successfully completed recombinant IL-1β was produced in a reliable quality and sufficient quantity. Monoclonal (mice) and polyclonal (sheep) antibodies against rabbit
INTRODUCTION Vaccines are used widely in the world. With the discovery of various vaccines mortality rate caused by various infectious organisms have reduced. Their use has been considered one of the safe and effective measures to control various infectious diseases. Plants offer enormous potential as production platforms for vaccines and therapeutic proteins. Vaccines derived from plants present an alternative to conventional vaccines by facilitating safe and effective oral delivery through intake
2013) The reaction processes at 10∼20MPa and 130∼160°C, when cobalt is used as the catalyst. The products ratio of n-butyraldehyde and isobutyraldehyde is 3. The reaction processes at 0.7-3MPa and 80-120°C, when rhodium is used as the catalyst. The products ratio of n-butyraldehyde and isobutyraldehyde of 8-16 can be achieved. The catalysts of nickel or copper in gaseous phase or nickel in liquid phase are used for hydrogenation. Second process is aldol condensation, in this reaction; two molecules