Enterococcus Faecalis Case Study

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INTRODUCTION Primary etiological factor responsible for pulpal and periapical diseases are microorganisms and their byproducts. Elimination of these microorganisms and their byproducts from the root canal can be achieved by biomechanical preparations. Mechanical instrumentation with hand or rotary files reduces significant amount of microflora, but due to complex anatomy of root canal system, microorganisms remaining in the intricacies of the canal can lead to pathology. Therefore, mechanical instrumentation of the root canal is accompanied by different root canal irrigants having antimicrobial property. According to current literature, sodium hypochlorite is most preferred root canal irrigant, due to its antimicrobial potential and tissue dissolution properties. However, it can lead to irritation of…show more content…
Brain heart infusion broth (Difco Co, Becton Dickinson, sparks, MD) supplemented with menadione (0.5 mg/l) and hemin (5 mg/l) was used to employed. P.guajava and Ocimum sanctum leaves were collected from the courtyard and washed with distilled water, later dried under the shade for 10-12 days. Dried leaves were then grounded into powder, in an electric grinder. Whatman filter paper no. 3 was used to pack the leaf powder and extracted under soxhlet extractor in methanol at 80 °C for 12 hour. Whatman filter paper no. 1 was used to filter the extract, and later dried using rota-vapour. Fruits of E. officinalis were dried for one month under shade and with the help of an electrical grinder grounded into powder. Powdered fruit material was extracted using Soxhlet extractor for 6 h using methanol as the solvent system not exceeding its boiling point. Whatman filter paper no. 1 was used to filter the extract, and later dried under reduced pressure using rota-vapour at 4° C for 10

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