Enterococcus Faecalis Lab Report

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Characteristics of Enterococcus faecalis Enterococcus faecalis is a type of bacteria which is non-motile and has a spherical shape. The genus name is known as Enterococcus due to it is found abundantly in large intestine of animals includes humans. Other than that, it can also found in large amount in our urinary tract and female reproductive system, vaginal. Whereas due to the Enterococcus faecalis is observed to be divided through binary fission across short chain in singly pairs, hence it is known as a sphere or cocci. Enterococcus faecalis basically is facultative anaerobe, they do not require any oxygen to grow in their medium but during their fermentative metabolism, Enterococcus faecalis can convert carbohydrate such as sugar into lactic acid. It is found out that during the conversion of sugar into lactic acid, Enterococcus faecalis do not produce any carbon dioxide but produce large amount of extracellular oxygen. In the laboratory, we can actually use gram stain technique to test Enterococcus faecalis. The result of the Enterococcus faecalis towards gram stain technique has shown that it is a gram-positive bacteria because it is stained with violet colour.…show more content…
The blood agar which is used to cultivate the Enterococcus faecalis usually is getting from the blood of animals such as sheep and it must be incubate at an aerobic condition at 37ºC within 24 hours. We will be able to get non-hemolytic (gamma-hemolytic) colonies of Enterococcus faecalis which is grown on the surface of blood agar in the next 24 hours of incubation. The growth colonies are greyish white in colour. Whereas the chocolate agar which is required for the growth of Enterococcus faecalis contains some additional nutrition require for the growth factor of Enterococcus faecalis. However, due to the chocolate agar do not contain precise nutrients; hence do not use it
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