Specific Restriction Enzyme Lab Report

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The first step in genetically modifying cotton is to remove the gene Cry1Ac from the bacteria Bacillus thuringiensis using a specific restriction enzyme. A restriction enzyme cuts DNA strands at highly specific sites, diagonally, leaving some of the nucleotides from each strand exposed on either end of the required gene. These are called ‘sticky ends’, making it easier to attach the gene into another piece of DNA. The second step is to prepare a bacterium (agrobacterium tumerfaciens) to carry the DNA into the host cell. A bacterial Ti plasmid (a small circular strand of DNA often found in many copies in bacteria in addition to their main DNA) is used as a vector (Figure 2). These replicate quickly and independently of the main bacterial genome,

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