MEASUREMENT OF SUCCINATE DEHYROGENASE ACTIVITY AND KINETICS IN ISOLATED MITOCHONDRIA
This exercise is about the measurement of succinate dehydrogenase activity and kinetics in isolated mitochondria.
I will first explain the functions of mitochondria in the cell before moving on to succinate dehydrogenase and how it functions in the citrate cycle.
Mitochondria refer to organelles in the cell which takes part in different kinds of metabolic functions. They are referred to as powerhouse of a cell because they provide the cell with the energy needed for growth and other life processes. ((C. Mary and F. Shawn (2009, 2012)).
Enzymes are biological catalysts that speed up the rate of chemical reaction. Without enzymes, substances that…show more content… Some tubes displayed the same colour change such as pink whiles other tubes displayed different colour changes such as purple or violet based on the substrate present in it.
The two practical’s followed similar procedure except the boiling of mitochondria in the measurement of succinate dehydrogenase practical at tube 7 whiles in practical 2 activity about succinate kinetics no mitochondria was boiled in any sample or tube.
In practical 1, the mitochondria were boiled at tube 7 to change the nature of succinate dehydrogenase and make it inactive to function. (P. Daniel (2009))
Also, I noticed different volumes of mitochondria suspension being used in table 1 (measurement of succinate) while in table 2 (study of kinetics of succinate dehydrogenase), the same volume of mitochondria suspension were used in all the samples or cuvettes. (P. Daniel (2009)).
Michaelis- Mentons enzyme kinetic equation states that initial velocity is directly proportional to enzyme concentration. The more enzyme presents, the greater the chances that more substrate will bind quicker to that enzyme because of the high affinity of enzymes for the substrate. (K. Gerald