Mimosa Pudica Case Study

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IN VITRO ANTIOXIDANT STUDIES OF LEAVES OF Mimosa pudica SANAYE M.M, JOGLEKAR C.S* Department of pharmacology, Prin.K.M.Kundnani college of pharmacy, cuffe parade colaba,Mumbai,400005 Abstract-Oxygen is one of the most important molecules on Earth mainly because of the biochemical symmetry of oxygenic photosynthesis and aerobic respiration that can maintain homeostasis within our planet’s biosphere. Oxygen can also produce toxic molecules, reactive oxygen species (ROS). ROS play a dual role in biological systems. They can be considered a double-edged sword because at moderate concentrations, nitric oxide (NO•), superoxide anion, and related reactive oxygen species play an important role as regulatory mediators in signalling processes. The present study was undertaken to…show more content…
The brain was quickly removed and chilled in ice cold phosphate buffer (pH 7.4) . After washing with ice cold phosphate buffer(pH 7.4), the brain was homogenized in phosphate buffer (pH 7.4) to get 10% brain homogenate. The reaction mixture contains 0.1 ml of sample, 0.2 ml of 8.1% sodium dodecyl sulfate (SDS), 1.5 ml of 20% acetic acid solution and 1.5 ml of 0.8% aqueous solution of thiobarbituric acid(TBA),20% acetic acid The mixture was finally made up to 4.0 ml with distilled water, and heated at 95°C for 60 min. After cooling with tap water, 1.0 ml of distilled water and 5.0 ml of mixture of n-butanol and pyridine (5: 1, v/v) were added, and the mixture was shaken vigorously. After centrifugation at 4000 rpm for 10 min, the absorbance of the organic layer (upper layer) was measured at 532 nm.[17][22] The radical scavenging activity was calculated from the equation:- % radical scavenging activity=(Abs control-Abs sample)/Abs control× 100 4.Total antioxidant capacity-

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