Lercanidipine was provided by the Sun Pharma, East Sikkim, and India. Ethyl cellulose, Hydroxyl Propyl Methyl Cellulose and Dibutylpthalate were obtained from Vikram Thermo, India. Oleic acid and Methanol were purchased from SD Fine Chemicals Ltd., Mumbai. All other chemicals and reagents were of analytical grade.
Preparation of lercanidipine transdermal patch:
Lercanidipine transdermal patches composed of different ratios of HPMC and Ethyl cellulose as shown in the Table 1 were prepared by solvent casting method. Accurately weighed polymers were soaked in distilled water for overnight. Dibutyl phthalate and oleic acid were added to this solution and mixed by means of magnetic stirrer. To this solution, lercanidipine dissolved in methanol was added and mixed for 30 min. This resulted solution was prepared in the form of patch by pouring in the Petridis containing mercury. The drying was carried out by placing in the hot air oven and maintained at 400c throughout the process.
Characterization of lercanidipine transdermal patch: Uniformity of patch weight:
This was done by weighing three different sides of the individual patch and the average weight was taken. This test was performed on…show more content… Sample was placed on a carbon specimen holder, and then coated with gold and then observed with a JOEL JSM-T330A scanning electron microscope.
Assay by UV:
A patch having 1 cm2 area was cut into small pieces and weighed. The patch was dissolved in 100 ml phosphate buffer saline of pH 7.4 by stirring with magnetic stirrer for 5 h. The content was filtered using 0.45 µm membrane filters and analyzed spectrophotometrically at 240 nm.
In vitro permeation across mice abdominal