Gel Electrophoresis Lab Report

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The purpose of this experiment was to determine the resolving ability of 0.8% agarose through gel electrophoresis. Three samples of lamda DNA were used; two of which were cut with restriction endonucleases. One of the samples was cut with restriction endonuclease HindIII, while the other sample was cut with restriction enzyme EcoRI. The remaining sample was uncut in order to act as a control throughout the experiment. Each sample was placed into a well composed of 0.8% agarose gel. Agarose gel was electrophoresed for 74 minutes in an electrophoresis chamber with a voltage of 2.7V/cm, containing a tris-borate-EDTA buffer solution. After 74 minutes, the gel was stained with Instastain Ethidium Bromide in order for DNA fragments to be seen under an ultraviolet transilluminator. Under the UV transilluminator, a total of 9 bands could be seen for DNA cut with HindIII, 5 bands for DNA cut with EcoRI, and 1 band for the uncut DNA. Rf values for each DNA sample were measured. The DNA sample cut with restriction endonuclease HindIII showed a total of 9 bands that had Rf values of 1.3, 1.5, 2.0, 2.5, 2.7, 3.2, 3.4, 5.0, and 5.2 cm. The actual base pair values were 23130, 9416, 6682, 4361, 3000, 2322, 2027, 725, and 564. A total of 5 bands were seen for the DNA sample that was cut with restriction endonuclease EcoRI. The…show more content…
The card was disposed into the waste bucket. The agarose gel was then placed under an Ultraviolet transilluminator in order to measure the distances the DNA fragments traveled. After closing the transilluminator, the UV light was turned on. The distances obtained represented the Rf value which shows how far the DNA fragments migrated. The Rf value was measured in centimeters using the ruler inside the UV transilluminator. After recording the Rf value, the light was turned off and the agarose gel was disposed into the trash. The gloves; however, were disposed into the waste

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