3.2.5 Agarose gel electrophoresis The 1X TBE will be needed and to get this concentration, the dilution from the given 10X TBE buffer will be organized. In demand to prepared 500 ml of 1X TBE, a volume of 450 ml of 1X TBE and 50 ml of 10X TBE will be required. The 1.0% concentration of agarose gel will be prepared by mix the agarose powder and 1X TBE buffer. A 1.0g of agarose powder will be weighed and suspended in 100 ml of 1X TBE buffer. The mixture will be completely dissolved by put in the oven
Objective: This lab was done to help understand restriction enzymes and how they work. As well as comprehend the correlation between these enzymes and DNA Fragments. This lab was conducted to estimate the size of unknown DNA fragment thru agarose gel and electrophoresis. Background: A restriction enzyme is a protein that recognizes a specific, short nucleotide sequence and cuts the DNA only at that specific site, which is known as restriction site or target sequence. Restriction enzymes
to our lab by Steep Hill Labs, Inc. We will be conducting our research is within the lab of Dr. Nolan Kane, under the supervision of Dr. Daniela Vergara and Dr. Nolan Kane. The Kane Lab’s
student researching microbial diversity based on macro genomics. I was able to do independently PCR and gel electrophoresis and learned what it was like to work in a busy lab. In another placement in China I was lucky enough to use some recombinant DNA technologies. Matt Ridley's 'Genome' widened my understanding of how hereditary information in humans directly affects certain traits. Having
Title of PhD research proposal: Morphological and Molecular Characterization, Micropropagation and nutritional assessment of cocoyam (Xanthosoma sagittifolium (L.) Schott) germplasms from Ethiopia By: Eyasu Wada Wachamo Email: eyasuwada@yahoo.com Phone number (Mobile): 251 912 841 769 Skype name: eyasu.wada1 Applied Genetics PhD student, Department of Microbial, Cellular and Moleulr Biology, Addis Ababa University 1. Introduction Cocoyam (Xanthosoma sagittifolium L. Schott) is herbaceous, perennial