Cellulose Lab Report

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2.1. Materials Microcrystalline cellulose was obtained from Sinopharm Chemical Reagent Co., Ltd, Shanghai, China. All other reagents were of analytical grade and used without pretreatment. 2.2. Preparation of oxidized cellulose 6.0 g of microcrystalline cellulose was dispersed in 100 ml of 2 M NaOH under stirring, and then 30 ml aliquot of epichlorohydrin(EDC) was added into the mixture continuing stirred for 20 h at 25oC. The product was filtered and washed with distilled water and acetone up to pH 7, dried at 90℃, and then stored for follow-up experiment. The epoxidized cellulose suspension (50 ml with a concentration of 8%) was mixed with 50 ml of aq. containing 7.9 g of NaIO4. The pH of the mixture was adjusted to 2.0 with 0.1M H2SO4.…show more content…
under shaking at 80℃and the pH was adjusted to 9 by NaOH solution. Then, 0.5 g oxidized cellulose was added into and continuing to react for 12 h. At the end of the reaction, 0.46 mmol NaBH4 dissolved in 10 ml aq. was added into the mixture and continue to react for 4 h. Finally, The product was washed repeatedly to neutral, and dried at 60℃. 2.4. Laccase…show more content…
The epoxy functions introduced in cellulose were used to anchor the melamine to cellulosic matrix through etherification reaction. The aldehyde functions were introduced in the epoxidized cellulose by partial oxidation with periodate, and resulted in the cleavage of the C2-C3 bond in the glucopyranose ring and the formation of two aldehyde groups per unit. The aldehyde groups could react with amino groups by Schiff-base formation, so the adjacent cellulose chains were crosslinked with melamine. The products were stabilized by a reduction process with sodium borohydride. Finally, laccase was covalently immobilized onto EDC-cellulose and EDC-Melamine cellulose (Scheme

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