Capillary Electrophoresis Lab

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Experiment 9: Capillary Electrophoresis- Microscale Separation of Vitamins Introduction In this experiment, an analysis was done on a mixture of vitamins using capillary electrophoresis in order to determine the concentrations of each. Capillary electrophoresis is a quantitative technique whereby a mixture of analytes, vitamins in this case, is separated based on their charge in an electric field. The vitamins being analyzed in this experiment were thiamine hydrochloride, nicotinic acid, and cyanocobalamin. Data and Observations Mass of nicotinic acid in 100 mL sodium phosphate buffer = 9.85 mg Mass of thiamine hydrochloride in 100 mL sodium phosphate buffer = 8.45 mg Mass of cyanocobalamin in 100 mL sodium phosphate buffer = 9.95 mg The…show more content…
(b) These calculations show that capillary electrophoresis is an incredibly efficient quantitative method in that it does not require a lot of analyte and can be run many times. If the experiment was run at negative polarity, the results would be opposite in the sense that the mobility of the species would be flipped such that nicotinic acid would be the quickest and the slowest would be thiamine hydrochloride. Electroosmosis would be decreased since the surface layer would repel the phosphate buffer. If one were looking at a positively charged constituent then too much buffer could interfere with it and the results would not be indicative of how much of the positively charged constituent was in the sample. If one were looking at a negatively charged sample, the results would be thrown off since the buffer would shield the negative charge and it would thus move more quickly in the

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