REPUBLIC OF THE PHILIPPINES
BATANGAS STATE UNIVERSITY
COLLEGE OF ENGINEERING, ARCHITECTURE AND FINE ARTS
INSTRUMENTATION AND CONTROL ENGINEERING (ICE) DEPARTMENT
VILLANO, MARY ANN B.
Capillary Electrophoresis and Capillary Electrochromatography
So first, here is a brief history about electrophoresis. The word electrophoresis came from the Greek words “electro” which means flow of electricity and “phoresis” means to carry across.
Electrophoresis is the motion of the dispersed particles relative to a fluid under the influence of spatiality uniform electric field. This electro kinetic phenomenon was observed in 1807 by Reuss.
Introduction…show more content… The electric field in turn is proportional to the magnitude of the applied voltage V and inversely proportional to the length L over which it is applied. Thus
V= Uₑ x (V/L)
This relationship indicates that high applied voltages are desirable to achieve rapid ionic migration and a fast separation. It is desirable to have rapid separations, but it is even more important to achieve high resolution separations. So we must examine the factors that determine resolution in electrophoresis.
Plate Heights in CE
In chromatography, both longitudinal diffusion and mass-transfer resistance contribute to band broadening. However, because only a single phase is used in electrophoresis, in theory only longitudinal diffusion needs to be considered. In practice, however, Joule heating can add variance as well as the injection process. Although CE is not a chromatographic process, separations are often described in a manncr similar to chromatography. For example, in electrophoresis, we caleulate the plate count N by