Leaf Chromatography Lab

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Abstract During the process of photosynthesis, plants capture a small fraction of the sun's energy and store it in the chemical bonds of carbohydrates. The light energy used is known as a visible wavelength in the light spectrums. These are the colors we see every day. In this experiment, we use a coin to transfer the juices of the a leaf onto chromatography paper which is then put into a solvent and used to separate the different pigments in the juices like yellow, orange, light green and dark green. In this experiment, it separates the two types of chlorophylls (chlorophylls A and B) the carotenes and xanthophylls. All of the pigments are non-polar to different levels while the solution the paper…show more content…
It was then placced in an acitone base and allowed to sit for a time until the pigmants had been given a chance to travel up the paper. Once the pigmants placement had been recorded each strips was cut to seperate the pigmants from each other. Those strips were placed in 5 indevial beakers labled. A1, A2, B, C and x. The pigmants sampled were placed in curvettes. Additional samples contains actitones were also created to be used as blanks as a control group for the experiement. The absobancy data was collected form the spectromiter and recorded onto a…show more content…
As for the color migration on the chromatography paper, After we placed the chromatography paper in the solution we determined that Chlorophyll B in the leave was the most polar while the carotenoids were the most nonpolar followed by the Xanthophylls and then Chlorophyll A. This experiment mostly exsisted to prove what we see when we see green plant or any thing really. For plants, green is not absorbed in the plant, while other colors like red, blue and violet are absorbed and used towards light energy for the plant to function. A very simple concept that can be used to help our understand of pigmeants and light

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