Enzymes: Chemical Degradation of Albumin through Light Absorbance Mariel Melchor, Itzae Facio, Sarahi Iturriaga, Sarai Chairez, Evelyn Tovanche University of Texas at El Paso BIOL 1107 October 9, 2014 Horacio Gonzalez Abstract In this enzyme lab, we worked to observe enzymatic activity by measuring how fast pepsin degrades albumin by observing the changes in absorbance of albumin using a spectrophotometer. The wavelength set through a spectrophotometer is the controlled variable
Enzymes An enzyme is a catalyst for a reaction. It causes the reaction to start or to speed up. A typical enzyme’s reaction is as follows: Enzymes are active in many reactions that occur every day. An example of this is the use of sucrose (an enzyme) in the breakdown of sucrose in human and other cells. Another example that is prevalent in plants is the reaction causing catechol to become benzoquinone; the catalyst for this is catecholase. This reaction happens when the plant is damaged. It helps
This aim of this experiment was to test the effects that Temperature, pH, Substrate and Enzyme concentration have on Phosphatase enzyme activity. Mung beans were used as they contain the Phosphatase enzyme which can be easily extracted through homogenising with water and filtering with muslin. Phenolphthalein is produced as a single product in the reaction which shows the amount of enzyme activity as a higher enzyme activity will produce more Phenolphthalein due to more organic phosphate being broken
Enzymes Enzyme Concentration and Enzyme Activity As the enzyme concentration increased while the substrate concentration and pH (using a pH 7 buffer) were held constant, the rate of reaction increased until there wasn’t an excess amount of substrate. Once this occurred, the reaction rate leveled off (and decreased due to human error). The absorbance of the wavelength (420nm) through the solution is proportional to the concentration of solute molecules and the reaction rate of the solution. When
Introduction Enzymes are proteins that act as catalysts for biological and chemical reactions; enzymes can be produced by a living organism or they can be man-made. Enzymes “speed up reactions by providing an alternative reaction pathway of lower activation energy”, but the enzymes are not consumed in the process (“Enzymes”). Enzymes form a complex with the substrate. It is possible that the enzyme may change when it is bound to the substrate, but it will go back to its original form after a while
Reacting at the Speed of Enzymes Alexandrea Cassidy Biology 1111 Section 7 Dr. Shazia Ahmed September 30, 2015 INTRODUCTION This experiment covered the role of enzymes in nature as catalysts to quicken reaction times and lower the energy required to begin a reaction (Ahmed 2005). The enzyme is considered a protein but it contains non protein parts named cofactors or coenzymes that are organic molecules. An enzyme is created in a specific shape with an area that is carved out called
Enzymes work in an assortment of environments such as varying enzyme concentrations, pHs, and temperatures. The enzyme used in the experiment, catalase, works in a variety of aerobic organisms. The enzyme formed a substrate with the hydrogen peroxide in order to break it down. Catalase works to breakdown toxic hydrogen peroxide in cells and creates a by-product of harmless water and oxygen. The A spectrophotometer was used to identify the indicator solution, guaiacol, added to the various solutions
Enzymes facilitate the reaction in a cell that make and break down polymers; enzymes are specialized macromolecules that speed up chemical reactions. Enzymes are specific to molecules they catalyze chemical reactions (Campbell Biology, 2014 pg 67). The active site can only fit a certain type of molecule, but many contribute to the completion of different enzymes speed up reactions (Goldsworthy, 73). Enzymes are important because they speed up chemical reactions that could otherwise take years to
The enzyme experiment The enzyme is a miraculous process necessary for many scientific explanations for life forms, and the overall existence of humanity. An enzyme, according to Geoffrey M. Cooper, is a “catalysts that [can] increase the rate of virtually all the chemical reactions within cells. Most biological reactions are catalyzed by proteins.” Thus the enzyme is made up of protein polypeptide bonds, and with the help of the enzyme, the chemical reaction can be sped up and occur within a few
Steven Bae Franckowiak AP Biology 26 September 2014 Enzyme Lab Introduction: Enzymes are proteins that speed up the rate of a certain chemical reactions. In the lab, the enzyme, catalase was studied. Catalase is essential to many living organisms. It helps convert hydrogen peroxide (H2O2) into water and oxygen. Hydrogen peroxide is powerful and not harmless, with any solution having more than eight percent hydrogen peroxide being corrosive to skin (Britannica). In everyday life, it can be used