Enzyme Lab Report

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Enzymes An enzyme is a catalyst for a reaction. It causes the reaction to start or to speed up. A typical enzyme’s reaction is as follows: Enzymes are active in many reactions that occur every day. An example of this is the use of sucrose (an enzyme) in the breakdown of sucrose in human and other cells. Another example that is prevalent in plants is the reaction causing catechol to become benzoquinone; the catalyst for this is catecholase. This reaction happens when the plant is damaged. It helps to prevent infection and disease in the wound of the plant. The reaction can be observed when an apple browns after it has been cut of bitten. The reaction is as follows: When the plant is injured, the cell vacuole is damaged. This releases the catechol into the cytoplasm where the catecholase is. When the two mix together with O2, they become benzoquinone and water. Catechol is clear, however, benzoquinone is reddish brown,…show more content…
The procedure for this section was similar to the procedure for measuring the control groups. Tube 1 was filled with 1 mL or the enzyme solution and 2 ml of EDTA. Tube 2 was filled with 1 mL enzyme solution and 2 ml PTU. Tube 3 was filled with 1 mL enzyme solution and 2 mL of citric acid. Tube 4 was filled with 1 mL enzyme solution and 2 ml dH2O. Tube 5 was the calibration tube and is filled only with 5 mL of dH2O. These solutions were mixed and sat at room temperature for 10 minutes total, being mixed at 2 minute intervals. After 10 minutes, 2 mL catechol was added to tubes 1, 2, 3, and 4. The tubes sat for 10 minutes to give the chelating agents time to bind with the ions. The same procedure for calibrating the spectrophotometer and measuring absorption rates of the tubes were repeated immediately after catechol was added to the solution. After 10 minutes repeat the same measuring procedure as seen in the initial procedure. Results were recorded in a

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