E. Coli Cell Transformation Lab Report

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Abstract The process of bacteria up taking foreign DNA from its environment was explored. Escherichia coli cells were made competent and heat shocked. The process of transformation was used to give Escherichia coli cells antibiotic resistance and bioluminescence. The E. coli cells that were successfully transformed and placed on an agar containing the ampicillin, survived and reproduced forming colonies. The E. coli with the lux operon only expressed bioluminescence when incubated on agar plate containing ampicillin. The E. coli cells that expressed ampicillin resistance did so by expressing the DNA in a control plasmid that coded for enzymes that deactivate an antibiotic function. The E. coli cells that bioluminescence did so because their plasmid contained the lux operon. The lux operon contained both bioluminescence genes and antibiotic resistance genes. The cell only expressed plasmids if it was necessary for its survival Therefore, these E. coli cells only expressed bioluminescence when placed on an agar contain ampicillin. Introduction Transformation was used in an effort make Escherichia coli cells antibiotic resistant and bioluminescent. The process of transformation involves the uptake of DNA from the environment (Raven et al., 2014). It has three requirements: a host, a vector, and a way of identifying the transformed cells (Alberte et al., 2012). The vectors used in this experiment were the plasmid vectors pUC18 and lux, both of which code for antibiotic resistance. Plasmids are…show more content…
This was done by placing both, a vial containing a CaCl2 solution and a tube containing 50 μL of Escherichia coli in an ice bath. Then a micropipette was used transfer 590 μL of the CaCl2 solution into the tube containing the E. coli. The tube was taken out of the ice bath, agitated slightly, then placed back into the ice bath for a minimum of 10 minutes to
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