Materials and Methods Restriction enzyme digestion is a method used to identify any differences in DNA sequences among people, the use of restriction enzymes are what help to determine the order of DNA in which they attach to recognition sites and cut the DNA. People with different genes have different recognition sites for restriction enzymes. (Upadhyaya 45-46) In this experiment, four test tubes were used for restriction enzyme digestion reactions which were all filled using a micropipette with
Extracted DNA are to be subjected to the action of enzymes to cleave the DNA 4. Gel Electrophoresis is to be performed to separate the DNA Fragments 5. The fragments are to be transferred to nitrocellulose membrane using Southern Blotting for separation 6. Radioactive probe are to be added to the membrane for binding to specific DNA 7. Excess probe has to be removed by washing 8. To be Exposed to X-ray probe 9. To be Viewed under UV Transmitter for DNA Comparison 2.4. Advantages:
the restriction enzymes . This is accomplished by specific proteins known as polymerases, enzymes that are able to string together individual DNA building blocks to form long molecular strands. Through this process, multiple copies of the gene of interest can be produced. PCR proceeds in three stages, denaturation, annealing and extension. 2. What is the procedure of PCR ? 1. Denaturation The DNA template is heated to 94° C. This breaks the weak hydrogen bonds that hold DNA strands together
Genomics in the News DNA Profiling Rhys Kavanagh-Jones | ANiccol | TG: 22 DNA profiling is the use and storage of a DNA fingerprint. A DNA fingerprint can be used to identify people and when coupled with the raw capability and processing power of a database• the DNA profile becomes a tool for many to wield. DNA profiling can be used to solve crimes, track people and even perform paternity tests. • (Science Museum of London, DNA Profiling) Regardless of affinity DNA Profiling will affect everyone