Introduction To meet the increasing demand of robust, high turnover, easily and economically available biocatalyst, research is always channelized to get novelty in enzyme or improvement of existing enzymes by engineering at gene and protein level [1]. Enzymes derived from microbial source are generally regarded as safe and they are functional at wide range of temperature, pH, salinity or other extreme conditions. Actinomycetes are one of the most diverse groups of microorganisms that are well characterized
In this instance too there is a sense of alienation from self: the person in the mirror is very different from the person with the blades. And the most insightful moment of the sense of this alienation and separation occurs in the hospital after her suicide attempt with sleeping pills. Her face is distorted to such an extent that she is literally unrecognizable to herself. When she asks for a mirror to the nurse inside the ward, the nurse refuses to hand it to her, but when she insists, a mirror